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Journal of Clinical Microbiology, November 2001, p. 4149-4151, Vol. 39, No. 11
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.11.4149-4151.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Evaluation of a Latex Agglutination Test (MRSA-Screen) for Detection of Oxacillin Resistance in Coagulase-Negative Staphylococci

L. Louie,* A. Majury, J. Goodfellow, M. Louie, and A. E. Simor

Department of Microbiology, Sunnybrook and Women's College Health Sciences Centre, and the University of Toronto, Toronto, Ontario, Canada

Received 16 January 2001/Returned for modification 26 June 2001/Accepted 12 August 2001

The MRSA-Screen (Denka-Seiken, Tokyo, Japan) latex agglutination test was evaluated for its ability to detect PBP 2a from 200 clinical isolates of coagulase-negative staphylococci (CoNS; 84 mecA-positive strains and 116 mecA-negative strains) consisting of 108 Staphylococcus epidermidis, 37 S. saprophyticus, 15 S. haemolyticus, 11 S. hominis, 10 S. capitis, 10 S. warneri, and 3 S. lugdunensis species as well as 6 other species of CoNS. The assay was compared with susceptibility testing with an agar screen plate with oxacillin at 6 µg/ml (OXA6), by oxacillin disk diffusion (DD), by broth microdilution (BMDIL), by the E test, and with Vitek GPS-SV and Vitek GPS-107 susceptibility cards. PCR for the detection of the mecA gene was used as the "gold standard." The sensitivities and specificities for the methods evaluated were as follows: MRSA-Screen, 100 and 100%, respectively; OXA6, 100 and 99%, respectively; DD, 98 and 62%, respectively; BMDIL, 100 and 60%, respectively; E test, 100 and 51%, respectively; Vitek GPS-SV susceptibility card, 98 and 87%, respectively; and Vitek GPS-107 susceptibility card, 100 and 61%, respectively. The MRSA-Screen test accurately and rapidly detected oxacillin resistance in CoNS.

* Corresponding author. Mailing address: Department of Microbiology, Sunnybrook and Women's College Health Sciences Centre, B121-2075 Bayview Ave., Toronto, Ontario, Canada M4N 3M5. Phone: (416) 480-4242. Fax: (416) 480-6845. E-mail: lisa.louie{at}swchsc.on.ca.

Journal of Clinical Microbiology, November 2001, p. 4149-4151, Vol. 39, No. 11
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.11.4149-4151.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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