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Journal of Clinical Microbiology, November 2001, p. 4155-4159, Vol. 39, No. 11
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.11.4155-4159.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Bacterial Genetic Fingerprint: a Reliable Factor in the Study of the Epidemiology of Human Campylobacter Enteritis?

B. Steinbrueckner,1,* F. Ruberg,2 and M. Kist2

Institut für Labormedizin, Klinikum Ingolstadt, D-85049 Ingolstadt,1 and Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum Freiburg, D-79104 Freiburg,2 Germany

Received 29 January 2001/Returned for modification 11 March 2001/Accepted 11 August 2001

The rate of human intestinal infections with more than a single Campylobacter strain was determined and the genetic variabilities of Campylobacter strains throughout an infection episode were investigated by means of pulsed-field gel electrophoresis (PFGE) and enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR). For 48 and 49 of 50 patients, all isolates from one sample showed identical patterns by PFGE and ERIC-PCR, respectively. Throughout an infection episode in 47 of 52 patients, the PFGE fingerprints of the isolates remained stable, while in 1 patient two different species were observed and in 4 patients different patterns were observed. Therefore, ERIC-PCR proved less discriminative than PFGE. These findings suggest that human infection with more than one Campylobacter strain is rare and should not significantly impair epidemiologic analyses. However, changes in the genetic fingerprint throughout an infection should be considered in the assessment of epidemiologic studies of Campylobacter spp.

* Corresponding author. Mailing address: Institut für Labormedizin, Klinikum Ingolstadt, Krumenauerstr. 25, D-85049 Ingolstadt, Germany. Phone: 49 (841) 880 2904. Fax: 49 (841) 880 2912. E-mail: bernhard.steinbrueckner{at}klinikum.ingolstadt.de.

Journal of Clinical Microbiology, November 2001, p. 4155-4159, Vol. 39, No. 11
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.11.4155-4159.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.