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Journal of Clinical Microbiology, November 2001, p. 4166-4168, Vol. 39, No. 11
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.11.4166-4168.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Application of a Simple Multiplex PCR To Aid in Routine Work of the Mycobacterium Reference Laboratory

Dorothy Yeboah-Manu,1 Malcolm D. Yates,2 and Stuart Mark Wilson2,*

Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana,1 and Public Health Laboratory Service Mycobacterium Reference Unit, Dulwich Hospital, East Dulwich Grove, East Dulwich, London SE22 8QF, United Kingdom2

Received 3 May 2001/Returned for modification 19 June 2001/Accepted 3 September 2001

A PCR specific for spacer regions 33 and 34 of the direct repeat region of the Mycobacterium tuberculosis complex was developed to complement the biochemical differentiation of M. tuberculosis, Mycobacterium bovis, M. bovis BCG, and Mycobacterium africanum subtypes I and II. In addition, this approach was incorporated into a multiplex PCR that included primers specific for IS6110 and the 65-kDa antigen gene in order to differentiate members of the M. tuberculosis complex from atypical mycobacteria.

* Corresponding author. Present address: Microsens Biophage Ltd., London Bioscience Innovation Centre, 2 Royal College St., London NW1 0TU, United Kingdom. Phone: 020 7691 2147. Fax: 0870 054 8341. E-mail: stuart{at}microsens.demon.co.uk.

Journal of Clinical Microbiology, November 2001, p. 4166-4168, Vol. 39, No. 11
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.11.4166-4168.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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