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Journal of Clinical Microbiology, November 2001, p. 4181-4183, Vol. 39, No. 11
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.11.4181-4183.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Trends in Antifungal Susceptibility among Swedish Candida Species Bloodstream Isolates from 1994 to 1998: Comparison of the E-test and the Sensititre YeastOne Colorimetric Antifungal Panel with the NCCLS M27-A Reference Method

Erja Chryssanthou*

Department of Clinical Microbiology, Karolinska Hospital, Stockholm, Sweden

Received 16 April 2001/Returned for modification 30 July 2001/Accepted 30 August 2001

A comparative evaluation of the NCCLS macrodilution method, the E-test, and the Sensititre YeastOne Colorimetric Antifungal Panel for the susceptibility testing of fluconazole, itraconazole, amphotericin B, and flucytosine was conducted with 233 blood isolates of Candida species collected between 1994 and 1998 in Sweden. Antifungal susceptibility profiles of Candida albicans and non-C. albicans Candida species remained essentially unchanged within the 5-year study period. The overall agreement rates for the E-test and the NCCLS MICs and for the YeastOne and the NCCLS MICs were >= 86 and >= 87%, respectively, within ±1 dilution for fluconazole, amphotericin B, and flucytosine, and >= 66 and >= 57%, respectively, for itraconazole. The E-test and the YeastOne panels are equivalent, and both are convenient methods for routine use.

* Mailing address: Department of Clinical Microbiology, L202 Karolinska Hospital, S-171 76 Stockholm, Sweden. Phone: (46) 851773566. Fax: (46) 8308099. E-mail: Erja.Chryssanthou{at}ks.se.

Journal of Clinical Microbiology, November 2001, p. 4181-4183, Vol. 39, No. 11
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.11.4181-4183.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.