JCM Figure table search 04
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Menendez, M. C.
Right arrow Articles by Garcia, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Menendez, M. C.
Right arrow Articles by Garcia, M. J.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, December 2001, p. 4241-4246, Vol. 39, No. 12
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.12.4241-4246.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Characterization of a Mycobacterium intracellulare Variant Strain by Molecular Techniques

M. C. Menendez,1 E. Palenque,2 M. C. Navarro,1 M. C. Nuñez,1 M. J. Rebollo,1 and M. J. Garcia1,*

Departamento de Medicina Preventiva, Facultad de Medicina, Universidad Autonoma de Madrid, 28029 Madrid,1 and Laboratorio de Micobacterias, Servicio de Microbiologia, Hospital Universitario 12 de Octubre, 28041 Madrid,2 Spain

Received 26 April 2001/Returned for modification 5 August 2001/Accepted 8 September 2001

This paper describes a Mycobacterium intracellulare variant strain causing an unusual infection. Several isolates obtained from an immunocompromised patient were identified as members of the Mycobacterium avium complex (MAC) by the commercial AccuProbe system and biochemical standard identification. Further molecular approaches were undertaken for a more accurate characterization of the bacteria. Up to seven different genomic sequences were analyzed, ranging from conserved mycobacterial genes such as 16S ribosomal DNA to MAC-specific genes such as mig (macrophage-induced gene). The results obtained identify the isolates as a variant of M. intracellulare, an example of the internal variability described for members of the MAC, particularly within that species. The application of other molecular approaches is recommended for more accurate identification of bacteria described as MAC members.

* Corresponding author. Mailing address: Departamento de Medicina Preventiva, Facultad de Medicina, Universidad Autonoma de Madrid, St/ Arzobispo Morcillo s/n, 28029 Madrid, Spain. Phone: 34-91-397.5440. Fax: 34-91-397.5353. E-mail: mariaj.garcia{at}uam.es.

Journal of Clinical Microbiology, December 2001, p. 4241-4246, Vol. 39, No. 12
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.12.4241-4246.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 2001 by the American Society for Microbiology. All rights reserved.