Viral Load of Human Herpesvirus 8 in Peripheral Blood of Human Immunodeficiency Virus-Infected Patients with Kaposi's Sarcoma

doi:10.1128/JCM.39.12.4269-4273.2001

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Journal of Clinical Microbiology, December 2001, p. 4269-4273, Vol. 39, No. 12
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.12.4269-4273.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Viral Load of Human Herpesvirus 8 in Peripheral Blood of Human Immunodeficiency Virus-Infected Patients with Kaposi's Sarcoma

Rosamaria Tedeschi,¹^,²^,^* Malin Enbom,³ Ettore Bidoli,⁴ Annika Linde,³ Paolo De Paoli,² and Joakim Dillner¹^,⁵

The Microbiology and Tumor Biology Center¹ and Swedish Institute for Infectious Diseases Control,³ Karolinska Institute, Stockholm, and Department of Medical Microbiology, MAS University Hospital, Malmö,⁵ Sweden, and Microbiology-Immunology and Virology Department² and Epidemiology Unit,⁴ Centro di Riferimento Oncologico, IRCCS, Aviano, Italy

Received 25 June 2001/Returned for modification 21 July 2001/Accepted 10 September 2001

Viral load is an important marker of activity of viral diseases for a number of viruses. We wished to evaluate whether theviral load of human herpesvirus 8 (HHV-8) in peripheral bloodwas a consistent feature of Kaposi's sarcoma (KS) patients andwhether the viral load correlated with human immunodeficiencyvirus (HIV) RNA levels, CD4 counts, and/or the HHV-8 seroreactivity.Fifty-four consecutive plasma samples from 14 patients with KSwere evaluated for HHV-8 viral load by quantitative real-timePCR. Samples were analyzed at the start of highly active antiretroviraltherapy (HAART) and at different intervals during treatments.The median HHV-8 DNA load before HAART treatment was 8,998 (rangingfrom 170 to 40,100) copies/ml and 12,270 (ranging from 40 to 142,575)copies/ml during HAART. There were both increasing and decreasingtrends. There was an association between HHV-8 DNA and HIV RNAviral loads (odds ratio [OR] = 5.40; 95% confidence interval [95%CI], 1.54 to 18.98) and between HHV-8 viral load and CD4 cellcounts (OR = 7.24; 95% CI, 1.30 to 40.35). High HHV-8 viral loadwas also correlated with the titers of antibodies to the lyticHHV-8 antigen detected with immunofluorescence (P < 0.01), butnot with antibodies to the latent HHV-8 antigen. In conclusion,we found that HHV-8 viremia in KS is associated with HIV viralload, CD4 cell counts, and lytic HHV-8 serological reactivity.HHV-8 viral load monitored by real time PCR might be useful fordetermination HHV-8 viral load during the follow-up of KSpatients.

^* Corresponding author. Mailing address: Microbiology-Immunology and Virology Department, Centro di Riferimento Oncologico,IRCCS, Aviano, Italy. Phone: 39-0434-659421. Fax: 39-0434-659402.E-mail: rtedeschi{at}cro.it.

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