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Journal of Clinical Microbiology, December 2001, p. 4452-4455, Vol. 39, No. 12
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.12.4452-4455.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Identification of Pandoraea Species by 16S Ribosomal DNA-Based PCR Assays

Tom Coenye,1,* Lixia Liu,1 Peter Vandamme,2 and John J. LiPuma1

Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, Michigan,1 and Laboratory of Pharmaceutical Microbiology, Ghent University, Ghent, Belgium2

Received 14 June 2001/Returned for modification 4 September 2001/Accepted 17 September 2001

The recently described genus Pandoraea contains five named species (Pandoraea apista, Pandoraea pulmonicola, Pandoraea pnomenusa, Pandoraea sputorum, and Pandoraea norimbergensis) and four unnamed genomospecies. Pandoraea spp. have mainly been recovered from the respiratory tracts of cystic fibrosis (CF) patients. Accurate genus- and species-level identification by routine clinical microbiology methods is difficult, and differentiation from Burkholderia cepacia complex organisms may be especially problematic. This can have important consequences for the management of CF patients. On the basis of 16S ribosomal DNA sequences, PCR assays for the identification of Pandoraea spp. were developed. A first PCR assay was developed for the identification of Pandoraea isolates to the genus level. PCR assays for the identification of P. apista and P. pulmonicola as a group, P. pnomenusa, P. sputorum, and P. norimbergensis were also developed. All five assays were evaluated with a panel of 123 bacterial isolates that included 69 Pandoraea sp. strains, 24 B. cepacia complex strains, 6 Burkholderia gladioli strains, 9 Ralstonia sp. strains, 5 Alcaligenes xylosoxidans strains, 5 Stenotrophomonas maltophilia strains, and 5 Pseudomonas aeruginosa strains. The use of these PCR assays facilitates the identification of Pandoraea spp. and avoids the misidentification of a Pandoraea sp. as a B. cepacia complex isolate.

* Corresponding author. Mailing address: Department of Pediatrics and Communicable Diseases, 8301 MSRB III, Box 0646, 1150 W. Med. Ctr. Dr., Ann Arbor, MI 48109-0646. Phone: (734) 936-9767. Fax: (734) 615-4770. E-mail: tcoenye{at}umich.edu and tomcoenye{at}hotmail.com.

Journal of Clinical Microbiology, December 2001, p. 4452-4455, Vol. 39, No. 12
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.12.4452-4455.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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