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Journal of Clinical Microbiology, December 2001, p. 4462-4467, Vol. 39, No. 12
Research Center for Pathogenic Fungi and
Microbial Toxicoses, Chiba University, 1-8-1, Inohana, Chuo-ku,
Chiba 260-8673, Japan,1 and Department
of Pathogenobiology, Norman Bethune Medical School, Jilin
University, Changchun, Jilin 130021, China2
Received 2 July 2001/Returned for modification 8 September
2001/Accepted 29 September 2001
We analyzed a 402-bp sequence of the mitochondrial
cytochrome b gene of 34 strains of Exophiala
jeanselmei and 16 strains representing 12 related species. The
strains of E. jeanselmei were classified
into 20 DNA types and 17 amino acid types. The differences between
these strains were found in 1 to 60 nucleotides and 1 to 17 amino
acids. On the basis of the identities and similarities of nucleotide
and amino acid sequences, some strains were reidentified: i.e., two
strains of E. jeanselmei var.
hetermorpha and one strain of E.
castellanii as E.
dermatitidis (including the type strain), three strains
of E. jeanselmei as E.
jeanselmei var. lecanii-corni (including
the type strain), three strains of E.
jeanselmei as E. bergeri
(including the type strain), seven strains of E.
jeanselmei as E.
pisciphila (including the type strain), seven strains of E. jeanselmei as E.
jeanselmei var. jeanselmei (including the type strain), one strain of E. jeanselmei
as Fonsecaea pedrosoi (including the type strain), and
one strain of E. jeanselmei as E. spinifera (including the type strain).
Some E. jeanselmei strains showed
distinct nucleotide and amino acid sequences. The amino-acid-based UPGMA (unweighted pair group method with the arithmetic mean) tree
exhibited nearly the same topology as those of the DNA-based trees
obtained by neighbor joining, maximum parsimony, and maximum likelihood methods.
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.12.4462-4467.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Identification, Classification, and Phylogeny of
the Pathogenic Species Exophiala jeanselmei and Related
Species by Mitochondrial Cytochrome b Gene
Analysis
*
Corresponding author. Mailing address: Research Center
for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1-8-1, Inohana, Chuo-ku, Chiba 260-8673, Japan. Phone: 81-43-226-2789. Fax: 81-43-226-2486. E-mail:
yoko{at}myco.pf.chiba-u.ac.jp.
Journal of Clinical Microbiology, December 2001, p. 4462-4467, Vol. 39, No. 12
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.12.4462-4467.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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