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Journal of Clinical Microbiology, December 2001, p. 4472-4476, Vol. 39, No. 12
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.12.4472-4476.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Comparative Quantitation of Cytomegalovirus (CMV) DNA in Solid Organ Transplant Recipients with CMV Infection by Using Two High-Throughput Automated Systems

Raymund R. Razonable,1 Robert A. Brown,1 Mark J. Espy,2 Antonio Rivero,1 Walter Kremers,3,4 Jennie Wilson,4 Cynthia Groettum,4 Thomas F. Smith,2,* and Carlos V. Paya1,4,*

Division of Infectious Diseases and Internal Medicine,1 Department of Pathology and Laboratory Medicine,2 Section of Biostatistics,3 and Transplant Center,4 Mayo Clinic, Rochester, Minnesota

Received 19 June 2001/Returned for modification 21 July 2001/Accepted 8 October 2001

Cytomegalovirus (CMV) DNA quantitation in clinical specimens is progressively becoming a cornerstone in the diagnosis and management of CMV infection in the immunocompromised host. We evaluated two automated and reproducible PCR tests, the LightCycler (Roche Molecular Biochemicals, Indianapolis, Ind.) and the COBAS AMPLICOR CMV Monitor (Roche Diagnostics, Pleasanton, Calif.), for the detection of CMV DNA in blood samples from transplant recipients with CMV infection as determined by shell vial culture. Following a log transformation analysis, the mean CMV DNA in plasma (PL), whole blood (WB), peripheral blood leukocytes (PBL), and peripheral blood mononuclear cells (PBMC) using the LightCycler was 6.79 copies per ml, 7.23 copies per ml, 6.38 copies per 2 × 106 cells, and 6.27 copies per 2 × 106 cells, respectively. This compares to 7.86 copies per ml, 8.37 copies per ml, 7.59 copies per 2 × 106 cells, and 7.44 copies per 2 × 106 cells, respectively, using COBAS AMPLICOR CMV Monitor. While higher CMV DNA levels were observed for the various blood compartments analyzed using COBAS AMPLICOR CMV Monitor, a high degree of correlation was evident between the two automated systems (jackknife correlation r = PL 0.77 [95% confidence interval (CI); 0.64, 0.90], WB 0.77 [95% CI; 0.62, 0.92], PBL 0.77 [95% CI; 0.67, 0.88], and PBMC 0.81 [95% CI; 0.72, 0.89], all P < 0.001). Therefore, we conclude that either automated diagnostic system is accurate for CMV DNA quantitation.

* Corresponding author. Mailing address for Thomas F. Smith: Department of Pathology and Laboratory Medicine, Mayo Clinic, 200 First St. SW, Rochester, MN 55905. Phone: (507) 284-3747. Fax: (507) 284-3757. E-mail: tfsmith{at}mayo.edu. Mailing address for Carlos V. Paya: Division of Infectious Diseases, Mayo Clinic, 200 First St. SW, Rochester, MN 55905. Phone: (507) 284-3747. Fax: (507) 284-3757. E-mail: paya{at}mayo.edu.

Journal of Clinical Microbiology, December 2001, p. 4472-4476, Vol. 39, No. 12
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.12.4472-4476.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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