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Journal of Clinical Microbiology, December 2001, p. 4472-4476, Vol. 39, No. 12
Division of Infectious Diseases and Internal
Medicine,1 Department of Pathology and
Laboratory Medicine,2 Section of
Biostatistics,3 and Transplant
Center,4 Mayo Clinic, Rochester, Minnesota
Received 19 June 2001/Returned for modification 21 July
2001/Accepted 8 October 2001
Cytomegalovirus (CMV) DNA quantitation in clinical specimens is
progressively becoming a cornerstone in the diagnosis and management of
CMV infection in the immunocompromised host. We evaluated two automated
and reproducible PCR tests, the LightCycler (Roche Molecular
Biochemicals, Indianapolis, Ind.) and the COBAS AMPLICOR CMV Monitor
(Roche Diagnostics, Pleasanton, Calif.), for the detection of CMV DNA
in blood samples from transplant recipients with CMV infection as
determined by shell vial culture. Following a log transformation
analysis, the mean CMV DNA in plasma (PL), whole blood (WB), peripheral
blood leukocytes (PBL), and peripheral blood mononuclear cells (PBMC)
using the LightCycler was 6.79 copies per ml, 7.23 copies per ml, 6.38 copies per 2 × 106 cells, and 6.27 copies per 2 × 106 cells, respectively. This compares to 7.86 copies
per ml, 8.37 copies per ml, 7.59 copies per 2 × 106
cells, and 7.44 copies per 2 × 106 cells,
respectively, using COBAS AMPLICOR CMV Monitor. While higher CMV DNA
levels were observed for the various blood compartments analyzed using
COBAS AMPLICOR CMV Monitor, a high degree of correlation was evident
between the two automated systems (jackknife correlation r = PL 0.77 [95% confidence interval (CI); 0.64, 0.90], WB 0.77 [95% CI; 0.62, 0.92], PBL 0.77 [95% CI; 0.67, 0.88], and PBMC 0.81 [95% CI; 0.72, 0.89], all P < 0.001).
Therefore, we conclude that either automated diagnostic system is
accurate for CMV DNA quantitation.
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.12.4472-4476.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Comparative Quantitation of Cytomegalovirus (CMV)
DNA in Solid Organ Transplant Recipients with CMV Infection by Using
Two High-Throughput Automated Systems
*
Corresponding author. Mailing address for Thomas F. Smith: Department of Pathology and Laboratory Medicine, Mayo Clinic,
200 First St. SW, Rochester, MN 55905. Phone: (507) 284-3747. Fax: (507) 284-3757. E-mail: tfsmith{at}mayo.edu. Mailing address for Carlos V. Paya: Division of Infectious Diseases, Mayo Clinic, 200 First
St. SW, Rochester, MN 55905. Phone: (507) 284-3747. Fax: (507)
284-3757. E-mail: paya{at}mayo.edu.
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