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Journal of Clinical Microbiology, December 2001, p. 4483-4486, Vol. 39, No. 12
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.12.4483-4486.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Detection of Pathogenic Yersinia enterocolitica by a Rapid and Sensitive Duplex PCR Assay

Wim J. B. Wannet,* Michiel Reessink, Henk A. Brunings, and Henny M. E. Maas

National Institute of Public Health and the Environment, Bilthoven, The Netherlands

Received 19 June 2001/Returned for modification 23 July 2001/Accepted 17 September 2001

A duplex PCR assay targeting the ail and 16S rRNA genes of Yersinia enterocolitica was developed to specifically identify pathogenic Y. enterocolitica from pure culture. Validation of the assay was performed with 215 clinical Yersinia strains and 40 strains of other bacterial species. Within an assay time of 4 h, this assay offers a very specific, reliable, and inexpensive alternative to the conventional phenotypic assays used in clinical laboratories to identify pathogenic Y. enterocolitica.

* Corresponding author. Mailing address: National Institute of Public Health and the Environment (RIVM), P.O. Box 1, 3720 BA, Bilthoven, The Netherlands. Phone: (31) 30 274 2105. Fax: (31) 30 274 4418. E-mail: wim.wannet{at}rivm.nl.

Journal of Clinical Microbiology, December 2001, p. 4483-4486, Vol. 39, No. 12
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.12.4483-4486.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.