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Journal of Clinical Microbiology, December 2001, p. 4487-4494, Vol. 39, No. 12
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.12.4487-4494.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Genetic Diversity of Histoplasma capsulatum Strains Isolated from Soil, Animals, and Clinical Specimens in Rio de Janeiro State, Brazil, by a PCR-Based Random Amplified Polymorphic DNA Assay

Mauro de Medeiros Muniz,1 Claudia Vera Pizzini,1 José Mauro Peralta,2 Errol Reiss,3 and Rosely M. Zancopé-Oliveira1,*

Centro de Pesquisa Hospital Evandro Chagas, FIOCRUZ,1 and Universidade Federal do Rio de Janeiro,2 Rio de Janeiro, RJ, Brazil, and Centers for Disease Control and Prevention, Atlanta, Georgia3

Received 1 June 2001/Returned for modification 6 August 2001/Accepted 7 October 2001

Little is known about the genetic strain diversity and geographical range of Histoplasma capsulatum isolated in Rio de Janeiro State, Brazil. We characterized 13 environmental, 7 animal, and 28 clinical H. capsulatum isolates by using a PCR-based random amplified polymorphic DNA (RAPD) assay. DNA fingerprinting of these soil, animal, and clinical specimens was performed with four primers (1253, 1281, D-9355, and D-10513) and generated amplicons with considerable polymorphism. Although all of the isolates exhibited more than 80% genetic relatedness, they could be clustered into four to six genotypes for each primer. The RAPD profiles of H. capsulatum isolated from Rio de Janeiro State could be distinguished from those of the U.S. strains included in this study (Downs, G222B, G-186B, and FLS1) by showing less than 70% similarity to each primer. The genetic polymorphisms between H. capsulatum strains isolated from animals and soil obtained in the same geographic areas were 100% similar, suggesting that an environmental microniche could be acting as a source of infection for animals and the local human population.

* Corresponding author. Mailing address: Serviço de Micologia do Centro de Pesquisa Hospital Evandro Chagas, Fundação Oswaldo Cruz. Av. Brasil 4365, Manguinhos, Rio de Janeiro 21045-900, Brazil. Phone: (55-21) 598-4282. Fax: (55-21) 590-9988. E-mail: zancope{at}cpqhec.fiocruz.br.

Journal of Clinical Microbiology, December 2001, p. 4487-4494, Vol. 39, No. 12
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.12.4487-4494.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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  • Guedes, H. L. d. M., Guimaraes, A. J., Muniz, M. d. M., Pizzini, C. V., Hamilton, A. J., Peralta, J. M., Deepe, G. S. Jr., Zancope-Oliveira, R. M. (2003). PCR Assay for Identification of Histoplasma capsulatum Based on the Nucleotide Sequence of the M Antigen. J. Clin. Microbiol. 41: 535-539 [Abstract] [Full Text]  


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