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Journal of Clinical Microbiology, December 2001, p. 4514-4517, Vol. 39, No. 12
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.12.4514-4517.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Identification of Mycobacterium avium subsp. paratuberculosis in Biopsy Specimens from Patients with Crohn's Disease Identified by In Situ Hybridization

Leonardo A. Sechi,1,* Mura Manuela,1 Tanda Francesco,2 Lissia Amelia,2 Solinas Antonello,3 Fadda Giovanni,4 and Zanetti Stefania1

Dipartimento di Scienze Biomediche, Sezione di Microbiologia Sperimentale e Clinica,1 Istituto di Anatomia e Istologia Patologica,2 and Istituto di Patologia Speciale Medica,3 Università degli studi di Sassari, 07100 Sassari, and Istituto di Microbiologia, Facoltà di Medicina e Chirurgia "Agostino Gemelli," Università Cattolica del Sacro Cuore, 00168 Rome,4 Italy

Received 15 June 2001/Returned for modification 26 August 2001/Accepted 24 September 2001

Crohn's disease is a chronic inflammatory disease of the gastrointestinal tract of unknown etiology. We report on the presence of cell wall-deficient Mycobacterium avium subsp. paratuberculosis in 35 of 48 paraffin-embedded tissue specimens from 33 patients with Crohn's disease by in situ hybridization with IS900 as a probe.

* Corresponding author. Mailing address: Dipartimento di Scienze Biomediche, Sezione di Microbiologia Sperimentale e Clinica, Università degli studi di Sassari, Viale S. Pietro 43/B, 07100 Sassari, Italy. Phone: 79 228303. Fax: 79 212345. E-mail: sechila{at}ssmain.uniss.it.

Journal of Clinical Microbiology, December 2001, p. 4514-4517, Vol. 39, No. 12
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.12.4514-4517.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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