This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Renesto, P.
Right arrow Articles by Raoult, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Renesto, P.
Right arrow Articles by Raoult, D.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, February 2001, p. 430-437, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.430-437.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Use of rpoB Gene Analysis for Detection and Identification of Bartonella Species

Patricia Renesto,1 Joanny Gouvernet,2 Michel Drancourt,1 Veronique Roux,1 and Didier Raoult1,*

Unité des Rickettsies, CNRS UPRES-A 6020, Faculté de Médecine, Université de la Méditerranée,1 and Service de l'Information Médicale, Hôpital de la Timone,2 13385 Marseille, France

Received 6 March 2000/Returned for modification 27 June 2000/Accepted 6 November 2000

Identification of Bartonella species is of increasing importance as the number of infections in which these bacteria are involved increases. To date, these gram-negative bacilli have been identified by various serological, biochemical, and genotypic methods. However, the development of alternative tools is required, principally to circumvent a major risk of contamination during sample manipulation. The aim of our study was to investigate the possible identification of various Bartonella species by comparison of RNA polymerase beta-subunit gene (rpoB) sequences. This approach has previously been shown to be useful for the identification of members of the family Enterobacteriaceae (C. M. Mollet, M. Drancourt, and D. Raoult, Mol. Microbiol. 26:1005-1011, 1997). Following PCR amplification with specific oligonucleotides, a 825-bp region of the rpoB gene was sequenced from 13 distinct Bartonella strains. Analysis of these sequences allowed selection of three restriction enzymes (ApoI, AluI, and AflIII) useful for discerning the different strains by PCR-restriction fragment length polymorphism (PCR-RFLP) analysis. To confirm the potential value of such an approach for identification of Bartonella, the rpoB PCR was then applied to 94 clinical samples, and the results obtained were identical to those obtained by our reference PCR method. Twenty-four isolates were also adequately identified by PCR-RFLP analysis. In all cases, our results were in accordance with those of the reference method. Moreover, conserved regions of DNA were chosen as suitable primer targets for PCR amplification of a 439-bp fragment which can be easily sequenced.

* Corresponding author. Mailing address: Unité des Rickettsies, CNRS UPRES-A 6020, Faculté de Médecine, Université de la Mediterranée, 13385 Marseille, France. Phone: 33 4 91 83 43 75. Fax: 33 4 91 83 03 90. E-mail: Didier.Raoult{at}medecine.univ-mrs.fr.

Journal of Clinical Microbiology, February 2001, p. 430-437, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.430-437.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Harrus, S., Bar-Gal, G. K., Golan, A., Elazari-Volcani, R., Kosoy, M. Y., Morick, D., Avidor, B., Baneth, G. (2009). Isolation and Genetic Characterization of a Bartonella Strain Closely Related to Bartonella tribocorum and Bartonella elizabethae in Israeli Commensal Rats. Am J Trop Med Hyg 81: 55-58 [Abstract] [Full Text]  
  • Henn, J. B., Gabriel, M. W., Kasten, R. W., Brown, R. N., Koehler, J. E., MacDonald, K. A., Kittleson, M. D., Thomas, W. P., Chomel, B. B. (2009). Infective Endocarditis in a Dog and the Phylogenetic Relationship of the Associated "Bartonella rochalimae" Strain with Isolates from Dogs, Gray Foxes, and a Human. J. Clin. Microbiol. 47: 787-790 [Abstract] [Full Text]  
  • Lin, J.-W., Chen, C.-Y., Chen, W.-C., Chomel, B. B., Chang, C.-C. (2008). Isolation of Bartonella species from rodents in Taiwan including a strain closely related to 'Bartonella rochalimae' from Rattus norvegicus. J Med Microbiol 57: 1496-1501 [Abstract] [Full Text]  
  • Inoue, K., Maruyama, S., Kabeya, H., Yamada, N., Ohashi, N., Sato, Y., Yukawa, M., Masuzawa, T., Kawamori, F., Kadosaka, T., Takada, N., Fujita, H., Kawabata, H. (2008). Prevalence and Genetic Diversity of Bartonella Species Isolated from Wild Rodents in Japan. Appl. Environ. Microbiol. 74: 5086-5092 [Abstract] [Full Text]  
  • Lydy, S. L., Eremeeva, M. E., Asnis, D., Paddock, C. D., Nicholson, W. L., Silverman, D. J., Dasch, G. A. (2008). Isolation and Characterization of Bartonella bacilliformis from an Expatriate Ecuadorian. J. Clin. Microbiol. 46: 627-637 [Abstract] [Full Text]  
  • Martini, M., Menandro, M. L., Mondin, A., Pasotto, D., Mazzariol, S., Lauzi, S., Stelletta, C. (2008). Detection of Bartonella bovis in a cattle herd in Italy. Vet Rec. 162: 58-59 [Full Text]  
  • Eremeeva, M. E., Gerns, H. L., Lydy, S. L., Goo, J. S., Ryan, E. T., Mathew, S. S., Ferraro, M. J., Holden, J. M., Nicholson, W. L., Dasch, G. A., Koehler, J. E. (2007). Bacteremia, Fever, and Splenomegaly Caused by a Newly Recognized Bartonella Species. NEJM 356: 2381-2387 [Abstract] [Full Text]  
  • Werner, J. A., Feng, S., Kasten, R. W., Hodzic, E., Chomel, B. B., Barthold, S. W. (2006). Cloning, Characterization, and Expression of Bartonella henselae p26.. CVI 13: 830-836 [Abstract] [Full Text]  
  • Oteo, J. A., Portillo, A., Santibanez, S., Blanco, J. R., Perez-Martinez, L., Ibarra, V. (2006). Cluster of Cases of Human Rickettsia felis Infection from Southern Europe (Spain) Diagnosed by PCR.. J. Clin. Microbiol. 44: 2669-2671 [Abstract] [Full Text]  
  • Fournier, P.-E., Suhre, K., Fournous, G., Raoult, D. (2006). Estimation of prokaryote genomic DNA G+C content by sequencing universally conserved genes.. Int. J. Syst. Evol. Microbiol. 56: 1025-1029 [Abstract] [Full Text]  
  • Khamis, A., Raoult, D., La Scola, B. (2004). rpoB Gene Sequencing for Identification of Corynebacterium Species. J. Clin. Microbiol. 42: 3925-3931 [Abstract] [Full Text]  
  • Woestyn, S., Olive, N., Bigaignon, G., Avesani, V., Delmee, M. (2004). Study of Genotypes and virB4 Secretion Gene of Bartonella henselae Strains from Patients with Clinically Defined Cat Scratch Disease. J. Clin. Microbiol. 42: 1420-1427 [Abstract] [Full Text]  
  • Drancourt, M., Bodaghi, B., Lepidi, H., Le Hoang, P., Raoult, D. (2004). Intraocular Detection of Bartonella henselae in a Patient with HLA-B27 Uveitis. J. Clin. Microbiol. 42: 1822-1825 [Abstract] [Full Text]  
  • Adekambi, T., Colson, P., Drancourt, M. (2003). rpoB-Based Identification of Nonpigmented and Late-Pigmenting Rapidly Growing Mycobacteria. J. Clin. Microbiol. 41: 5699-5708 [Abstract] [Full Text]  
  • Khamis, A., Colson, P., Raoult, D., Scola, B. L. (2003). Usefulness of rpoB Gene Sequencing for Identification of Afipia and Bosea Species, Including a Strategy for Choosing Discriminative Partial Sequences. Appl. Environ. Microbiol. 69: 6740-6749 [Abstract] [Full Text]  
  • Rolain, J. M., Gouriet, F., Enea, M., Aboud, M., Raoult, D. (2003). Detection by Immunofluorescence Assay of Bartonella henselae in Lymph Nodes from Patients with Cat Scratch Disease. CVI 10: 686-691 [Abstract] [Full Text]  
  • Ko, K. S., Kim, J.-M., Kim, J.-W., Jung, B. Y., Kim, W., Kim, I. J., Kook, Y.-H. (2003). Identification of Bacillus anthracis by rpoB Sequence Analysis and Multiplex PCR. J. Clin. Microbiol. 41: 2908-2914 [Abstract] [Full Text]  
  • Dillon, B., Valenzuela, J., Don, R., Blanckenberg, D., Wigney, D. I., Malik, R., Morris, A. J., Robson, J. M., Iredell, J. (2002). Limited Diversity among Human Isolates of Bartonella henselae. J. Clin. Microbiol. 40: 4691-4699 [Abstract] [Full Text]  
  • Zeaiter, Z., Liang, Z., Raoult, D. (2002). Genetic Classification and Differentiation of Bartonella Species Based on Comparison of Partial ftsZ Gene Sequences. J. Clin. Microbiol. 40: 3641-3647 [Abstract] [Full Text]  
  • Maurin, M., Rolain, J. M., Raoult, D. (2002). Comparison of In-House and Commercial Slides for Detection by Immunofluorescence of Immunoglobulins G and M against Bartonella henselae and Bartonella quintana. CVI 9: 1004-1009 [Abstract] [Full Text]  
  • Ko, K. S., Lee, H. K., Park, M.-Y., Lee, K.-H., Yun, Y.-J., Woo, S.-Y., Miyamoto, H., Kook, Y.-H. (2002). Application of RNA Polymerase {beta}-Subunit Gene (rpoB) Sequences for the Molecular Differentiation of Legionella Species. J. Clin. Microbiol. 40: 2653-2658 [Abstract] [Full Text]  
  • Ko, K. S., Lee, H. K., Park, M.-Y., Park, M.-S., Lee, K.-H., Woo, S.-Y., Yun, Y.-J., Kook, Y.-H. (2002). Population Genetic Structure of Legionella pneumophila Inferred from RNA Polymerase Gene (rpoB) and DotA Gene (dotA) Sequences. J. Bacteriol. 184: 2123-2130 [Abstract] [Full Text]  
  • Drancourt, M., Raoult, D. (2002). rpoB Gene Sequence-Based Identification of Staphylococcus Species. J. Clin. Microbiol. 40: 1333-1338 [Abstract] [Full Text]  
  • Zeaiter, Z., Fournier, P.-E., Raoult, D. (2002). Genomic Variation of Bartonella henselae Strains Detected in Lymph Nodes of Patients with Cat Scratch Disease. J. Clin. Microbiol. 40: 1023-1030 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»