This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Apfalter, P.
Right arrow Articles by Hirschl, A. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Apfalter, P.
Right arrow Articles by Hirschl, A. M.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, February 2001, p. 519-524, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.519-524.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Multicenter Comparison Trial of DNA Extraction Methods and PCR Assays for Detection of Chlamydia pneumoniae in Endarterectomy Specimens

Petra Apfalter,1,* Francesco Blasi,2 Jens Boman, Charlotte A. Gaydos,4 Michael Kundi,5 Matthias Maass,6 Athanasios Makristathis,1 Adam Meijer,7 Reinhard Nadrchal,1 Kenneth Persson,8 Manfred L. Rotter,1 C. Y. William Tong,9,dagger Gerold Stanek,10 and Alexander M. Hirschl1

Department of Clinical Microbiology,1 and Department of Infection Immunology,10 Hygiene-Institute, and Department of Occupational and Social Hygiene, Institute of Environmental Health,5 University of Vienna, Vienna, Austria; Institute of Respiratory Diseases, IRCCS Policlinico, University of Milan, Milan, Italy2; Department of Clinical Virology, Umeå University Hospital, Umeå, Sweden3; Division of Infectious Diseases, Department of Medicine, The Johns Hopkins University, Baltimore, Maryland4; Institute of Medical Microbiology, Medical University of Lübeck, Lübeck, Germany6; Research Laboratory for Infectious Diseases, National Institute of Public Health and the Environment, Bilthoven, The Netherlands7; Department of Clinical Virology, Malmö General Hospital, University of Lund, Lund, Sweden8; and Department of Medical Microbiology and Genitourinary Medicine, University of Liverpool, Liverpool, United Kingdom9

Received 16 August 2000/Returned for modification 11 November 2000/Accepted 25 November 2000

The reported rate of detection of Chlamydia pneumoniae DNA within atherosclerotic lesions by PCR varies between 0 and 100%. In this study, identical sets of coded experimental atheroma samples (n = 15) and spiked controls (n = 5) were analyzed by 16 test methods in nine centers by means of PCR. The positive controls were correctly identified to levels of 1, 0.1, and 0.01 inclusion bodies of C. pneumoniae/ml of tissue homogenate by 16 (100%), 11 (69%), and 3 (19%) of the test methods, respectively. Three out of 16 negative controls (19%) were rated positive. Positivity rates for atheroma samples varied between 0 and 60% for the different test methods, with the maximum concordant result for positivity being only 25% for one carotid artery sample. There was no consistent pattern of positive results among the various laboratories, and there was no correlation between the detection rates and the sensitivity of the assay used.

* Corresponding author. Mailing address: Department of Clinical Microbiology, Hygiene-Institute, University of Vienna, Vienna University Hospital, Währinger Gürtel 18-20/5P, A-1090 Vienna, Austria. Phone: 43 (1) 40400-5151. Fax: 43 (1) 40400-5228. E-mail: Petra.Apfalter{at}akh-wien.ac.at.

dagger Present address: Department of Infection, Virology Section, St. Thomas' Hospital, London SE 1 7EH, United Kingdom.

Journal of Clinical Microbiology, February 2001, p. 519-524, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.519-524.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Loens, K., Beck, T., Ursi, D., Pattyn, S., Goossens, H., Ieven, M. (2006). Two Quality Control Exercises Involving Nucleic Acid Amplification Methods for Detection of Mycoplasma pneumoniae and Chlamydophila pneumoniae and Carried Out 2 Years Apart (in 2002 and 2004).. J. Clin. Microbiol. 44: 899-908 [Abstract] [Full Text]  
  • Apfalter, P., Reischl, U., Hammerschlag, M. R. (2005). In-House Nucleic Acid Amplification Assays in Research: How Much Quality Control Is Needed before One Can Rely upon the Results?. J. Clin. Microbiol. 43: 5835-5841 [Full Text]  
  • Berg, H. F., Maraha, B., van der Zee, A., Gielis, S. K., Roholl, P. J. M., Scheffer, G.-J., Peeters, M. F., Kluytmans, J. A. J. W. (2005). Effect of Clarithromycin Treatment on Chlamydia pneumoniae in Vascular Tissue of Patients with Coronary Artery Disease: a Randomized, Double-Blind, Placebo-Controlled Trial. J. Clin. Microbiol. 43: 1325-1329 [Abstract] [Full Text]  
  • Ieven, M. M., Hoymans, V. Y. (2005). Involvement of Chlamydia pneumoniae in Atherosclerosis: More Evidence for Lack of Evidence. J. Clin. Microbiol. 43: 19-24 [Full Text]  
  • Pelton, S. I., Hammerschlag, M. R. (2005). Overcoming Current Obstacles in the Management of Bacterial Community-Acquired Pneumonia in Ambulatory Children. CLIN PEDIATR 44: 1-17  
  • Maraha, B., Berg, H., Kerver, M., Kranendonk, S., Hamming, J., Kluytmans, J., Peeters, M., van der Zee, A. (2004). Is the Perceived Association between Chlamydia pneumoniae and Vascular Diseases Biased by Methodology?. J. Clin. Microbiol. 42: 3937-3941 [Abstract] [Full Text]  
  • Apfalter, P., Barousch, W., Nehr, M., Willinger, B., Rotter, M., Hirschl, A. M. (2004). No Evidence of Involvement of Chlamydia pneumoniae in Severe Cerebrovascular Atherosclerosis by Means of Quantitative Real-Time Polymerase Chain Reaction. Stroke 35: 2024-2028 [Abstract] [Full Text]  
  • Hoymans, V. Y., Bosmans, J. M., Ursi, D., Martinet, W., Wuyts, F. L., Van Marck, E., Altwegg, M., Vrints, C. J., Ieven, M. M. (2004). Immunohistostaining Assays for Detection of Chlamydia pneumoniae in Atherosclerotic Arteries Indicate Cross-Reactions with Nonchlamydial Plaque Constituents. J. Clin. Microbiol. 42: 3219-3224 [Abstract] [Full Text]  
  • Esposito, S., Blasi, F., Bosis, S., Droghetti, R., Faelli, N., Lastrico, A., Principi, N. (2004). Aetiology of acute pharyngitis: the role of atypical bacteria. J Med Microbiol 53: 645-651 [Abstract] [Full Text]  
  • Hermann, C., Gueinzius, K., Oehme, A., von Aulock, S., Straube, E., Hartung, T. (2004). Comparison of Quantitative and Semiquantitative Enzyme-Linked Immunosorbent Assays for Immunoglobulin G against Chlamydophila pneumoniae to a Microimmunofluorescence Test for Use with Patients with Respiratory Tract Infections. J. Clin. Microbiol. 42: 2476-2479 [Abstract] [Full Text]  
  • Lindholt, J. S., Stovring, J., Ostergaard, L., Urbonavicius, S., Henneberg, E. W., Honore, B., Vorum, H. (2004). Serum Antibodies Against Chlamydia pneumoniae Outer Membrane Protein Cross-React With the Heavy Chain of Immunoglobulin in the Wall of Abdominal Aortic Aneurysms. Circulation 109: 2097-2102 [Abstract] [Full Text]  
  • Hardick, J., Maldeis, N., Theodore, M., Wood, B. J., Yang, S., Lin, S., Quinn, T., Gaydos, C. (2004). Real-Time PCR for Chlamydia pneumoniae Utilizing the Roche Lightcycler and a 16S rRNA Gene Target. J. Mol. Diagn. 6: 132-136 [Abstract] [Full Text]  
  • Goyal, M., Miller, S. T., Hammerschlag, M. R., Gelling, M., Gaydos, C. A., Hardick, J., Wood, B. J., Reznik, T., Rao, S.P. (2004). Is Chlamydia pneumoniae Infection Associated With Stroke in Children With Sickle Cell Disease?. Pediatrics 113: e318-e321 [Abstract] [Full Text]  
  • Apfalter, P., Hammerschlag, M. R., Boman, J., Wojta, J., Huber, K., Huk, I. (2003). Reliability of Nested PCR for the Detection of Chlamydia pneumoniae in Carotid Artery Atherosclerosis. Stroke 34 : e73-e75 [Full Text]  
  • Apfalter, P., Barousch, W., Nehr, M., Makristathis, A., Willinger, B., Rotter, M., Hirschl, A. M. (2003). Comparison of a New Quantitative ompA-Based Real-Time PCR TaqMan Assay for Detection of Chlamydia pneumoniae DNA in Respiratory Specimens with Four Conventional PCR Assays. J. Clin. Microbiol. 41: 592-600 [Abstract] [Full Text]  
  • Glader, C.A, Birgander, L.S, Soderberg, S, Ildgruben, H.P, Saikku, P, Waldenstrom, A, Dahlen, G.H (2003). Lipoprotein(a), Chlamydia pneumoniae, leptin and tissue plasminogen activator as risk markers for valvular aortic stenosis. Eur Heart J 24: 198-208 [Abstract] [Full Text]  
  • Kalayoglu, M. V., Libby, P., Byrne, G. I. (2002). Chlamydia pneumoniae as an Emerging Risk Factor in Cardiovascular Disease. JAMA 288: 2724-2731 [Abstract] [Full Text]  
  • Apfalter, P., Assadian, O., Blasi, F., Boman, J., Gaydos, C. A., Kundi, M., Makristathis, A., Nehr, M., Rotter, M. L., Hirschl, A. M. (2002). Reliability of Nested PCR for Detection of Chlamydia pneumoniae DNA in Atheromas: Results from a Multicenter Study Applying Standardized Protocols. J. Clin. Microbiol. 40: 4428-4434 [Abstract] [Full Text]  
  • Seemungal, T A R, Wedzicha, J A, MacCallum, P K, Johnston, S L, Lambert, P A, Blasi, F, Allegra, L, Damato, S, Raccanelli, R, Cosentini, R, Tarsia, P, Centanni, S (2002). Chlamydia pneumoniae and COPD exacerbation. Thorax 57: 1087-1089 [Full Text]  
  • Poppert, S., Essig, A., Marre, R., Wagner, M., Horn, M. (2002). Detection and Differentiation of Chlamydiae by Fluorescence In Situ Hybridization. Appl. Environ. Microbiol. 68: 4081-4089 [Abstract] [Full Text]  
  • Blasi, F, Damato, S, Cosentini, R, Tarsia, P, Raccanelli, R, Centanni, S, Allegra, L (2002). Chlamydia pneumoniae and chronic bronchitis: association with severity and bacterial clearance following treatment. Thorax 57: 672-676 [Abstract] [Full Text]  
  • Chernesky, M., Smieja, M., Schachter, J., Summersgill, J., Schindler, L., Solomon, N., Campbell, K., Campbell, L., Cappuccio, A., Gaydos, C., Chong, S., Moncada, J., Phillips, J., Jang, D., Wood, B. J., Petrich, A., Hammerschlag, M., Cerney, M., Mahony, J. (2002). Comparison of an Industry-Derived LCx Chlamydia pneumoniae PCR Research Kit to In-House Assays Performed in Five Laboratories. J. Clin. Microbiol. 40: 2357-2362 [Abstract] [Full Text]  
  • Yamamoto, Y. (2002). PCR in Diagnosis of Infection: Detection of Bacteria in Cerebrospinal Fluids. CVI 9: 508-514 [Full Text]  
  • Hermann, C., Graf, K., Groh, A., Straube, E., Hartung, T. (2002). Comparison of Eleven Commercial Tests for Chlamydia pneumoniae-Specific Immunoglobulin G in Asymptomatic Healthy Individuals. J. Clin. Microbiol. 40: 1603-1609 [Abstract] [Full Text]  
  • Saikku, P. (2002). Seroepidemiology in Chlamydia pneumoniae -- atherosclerosis association. Eur Heart J 23: 263-264 [Full Text]  
  • Tondella, M. L. C., Talkington, D. F., Holloway, B. P., Dowell, S. F., Cowley, K., Soriano-Gabarro, M., Elkind, M. S., Fields, B. S. (2002). Development and Evaluation of Real-Time PCR-Based Fluorescence Assays for Detection of Chlamydiapneumoniae. J. Clin. Microbiol. 40: 575-583 [Abstract] [Full Text]  
  • Boman, J., Hammerschlag, M. R. (2002). Chlamydia pneumoniae and Atherosclerosis: Critical Assessment of Diagnostic Methods and Relevance to Treatment Studies. Clin. Microbiol. Rev. 15: 1-20 [Abstract] [Full Text]  
  • Gaydos, C. A. (2001). Chlamydia pneumoniae and its proposed link to multiple sclerosis: To be or not to be?. Neurology 56: 1126-1127 [Full Text]  
  • Hammerschlag, M. R. (2001). Asymptomatic Respiratory Infection With Chlamydia pneumoniae : What Does It Mean?. Chest 119: 1303-1305 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»