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Journal of Clinical Microbiology, February 2001, p. 519-524, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.519-524.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Multicenter Comparison Trial of DNA Extraction Methods and PCR Assays for Detection of Chlamydia pneumoniae in Endarterectomy Specimens

Petra Apfalter,1,* Francesco Blasi,2 Jens Boman, Charlotte A. Gaydos,4 Michael Kundi,5 Matthias Maass,6 Athanasios Makristathis,1 Adam Meijer,7 Reinhard Nadrchal,1 Kenneth Persson,8 Manfred L. Rotter,1 C. Y. William Tong,9,dagger Gerold Stanek,10 and Alexander M. Hirschl1

Department of Clinical Microbiology,1 and Department of Infection Immunology,10 Hygiene-Institute, and Department of Occupational and Social Hygiene, Institute of Environmental Health,5 University of Vienna, Vienna, Austria; Institute of Respiratory Diseases, IRCCS Policlinico, University of Milan, Milan, Italy2; Department of Clinical Virology, Umeå University Hospital, Umeå, Sweden3; Division of Infectious Diseases, Department of Medicine, The Johns Hopkins University, Baltimore, Maryland4; Institute of Medical Microbiology, Medical University of Lübeck, Lübeck, Germany6; Research Laboratory for Infectious Diseases, National Institute of Public Health and the Environment, Bilthoven, The Netherlands7; Department of Clinical Virology, Malmö General Hospital, University of Lund, Lund, Sweden8; and Department of Medical Microbiology and Genitourinary Medicine, University of Liverpool, Liverpool, United Kingdom9

Received 16 August 2000/Returned for modification 11 November 2000/Accepted 25 November 2000

The reported rate of detection of Chlamydia pneumoniae DNA within atherosclerotic lesions by PCR varies between 0 and 100%. In this study, identical sets of coded experimental atheroma samples (n = 15) and spiked controls (n = 5) were analyzed by 16 test methods in nine centers by means of PCR. The positive controls were correctly identified to levels of 1, 0.1, and 0.01 inclusion bodies of C. pneumoniae/ml of tissue homogenate by 16 (100%), 11 (69%), and 3 (19%) of the test methods, respectively. Three out of 16 negative controls (19%) were rated positive. Positivity rates for atheroma samples varied between 0 and 60% for the different test methods, with the maximum concordant result for positivity being only 25% for one carotid artery sample. There was no consistent pattern of positive results among the various laboratories, and there was no correlation between the detection rates and the sensitivity of the assay used.


* Corresponding author. Mailing address: Department of Clinical Microbiology, Hygiene-Institute, University of Vienna, Vienna University Hospital, Währinger Gürtel 18-20/5P, A-1090 Vienna, Austria. Phone: 43 (1) 40400-5151. Fax: 43 (1) 40400-5228. E-mail: Petra.Apfalter{at}akh-wien.ac.at.

dagger Present address: Department of Infection, Virology Section, St. Thomas' Hospital, London SE 1 7EH, United Kingdom.


Journal of Clinical Microbiology, February 2001, p. 519-524, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.519-524.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.



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Copyright © 2001 by the American Society for Microbiology. All rights reserved.