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Journal of Clinical Microbiology, February 2001, p. 551-559, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.551-559.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Microsatellite Typing as a New Tool for Identification of Saccharomyces cerevisiae Strains

C. Hennequin,1,2,* A. Thierry,2 G. F. Richard,2 G. Lecointre,3 H. V. Nguyen,4 C. Gaillardin,4 and B. Dujon2

Service de Parasitologie-Mycologie et Médecine des Voyages, CHU Amiens, F-80054 Amiens,1 Unité de Génétique Moléculaire des Levures, URA 2171 CNRS and UFR 927, Université Pierre et Marie Curie, Paris, Institut Pasteur, F-75724 Paris,2 Service de Systematique Moléculaire (CNRS GDR 1005), Laboratoire d'Ichtyologie Generale et Appliquee, Museum National d'Histoire Naturelle, 75231 Paris Cedex 05,3 and Collection de Levures d'Intérêt Biotechnologique, UMR 216, Microbiologie et Génétique Moléculaire, Institut National d'Agronomie de Paris Grignon, F-78850 Thiverval Grignon,4 France

Received 25 July 2000/Returned for modification 14 August 2000/Accepted 12 October 2000

Since Saccharomyces cerevisiae appears to be an emerging pathogen, there is a need for a valuable molecular marker able to distinguish among strains. In this work, we investigated the potential value of microsatellite length polymorphism with a panel of 91 isolates, including 41 clinical isolates, 14 laboratory strains, and 28 strains with industrial relevance. Testing seven polymorphic regions (five trinucleotide repeats and two dinucleotide repeats) in a subgroup of 58 unrelated strains identified a total of 69 alleles (6 to 13 per locus) giving 52 different patterns with a discriminatory power of 99.03%. We found a cluster of clinical isolates sharing their genotype with a bakery strain, suggesting a digestive colonization following ingestion of this strain with diet. With the exception of this cluster of isolates and isolates collected from the same patient or from patients treated with Saccharomyces boulardii, all clinical isolates gave different and unique patterns. The genotypes are stable, and the method is reproducible. The possibility to make the method portable is of great interest for further studies using this technique. This work shows the possibility to readily identify S. boulardii (a strain increasingly isolated from invasive infections) using a unique and specific microsatellite allele.

* Corresponding author. Mailing address: Unité de Génétique Moléculaire des Levures, URA 2171 CNRS and UFR 927, Université Pierre et Marie Curie, Paris, Institut Pasteur, 25 rue du Dr. Roux, 75724 Paris, France. Phone: 33-1-45-68-07. Fax: 33-1-40-61-34-56. E-mail: chennequin{at}yahoo.com.

Journal of Clinical Microbiology, February 2001, p. 551-559, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.551-559.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.