This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow An erratum has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Oliveira, D. C.
Right arrow Articles by de Lencastre, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Oliveira, D. C.
Right arrow Articles by de Lencastre, H.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, February 2001, p. 574-580, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.574-580.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Comparison of DNA Sequencing of the Protein A Gene Polymorphic Region with Other Molecular Typing Techniques for Typing Two Epidemiologically Diverse Collections of Methicillin-Resistant Staphylococcus aureus

Duarte C. Oliveira,1,2 Inês Crisóstomo,1 Ilda Santos-Sanches,1,3 Peter Major,1,4 C. Rute Alves,1 Marta Aires-de-Sousa,1 Marianne K. Thege,4 and Hermínia de Lencastre1,2,*

Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal1; The Rockefeller University, New York, New York 100212; Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Monte da Caparica, Portugal3; and National Institute of Food Hygiene and Nutrition, Budapest, Hungary4

Received 19 October 2000/Returned for modification 25 November 2000/Accepted 1 December 2000

The aim of this study was to compare the recently developed typing approach for methicillin-resistant Staphylococcus aureus (MRSA) based on the DNA sequencing of the protein A gene polymorphic region (spaA typing) with a combination of three well-established molecular typing techniques: ClaI-mecA vicinity polymorphisms, ClaI-Tn554 insertion patterns, and SmaI pulsed-field gel electrophoresis (PFGE) profiles. In order to evaluate the applicability of this typing technique in different types of studies, two groups of MRSA clinical isolates were analyzed: a collection of 185 MRSA isolates circulating in Hungary recovered from 17 hospitals in seven cities during a 3-year period (1994 through 1996), and a selection of 53 MRSA strains isolated in a single hospital in Hungary between 1997 and 1998. The 238 MRSA clinical strains from Hungary were first classified in clonal types (defined as ClaI-mecA::ClaI-Tn554::SmaI-PFGE patterns), and 65 of the 238 strains, representing major MRSA clones and some sporadic clones, were further analyzed by spaA typing. Our results showed that the lineages most recently introduced in the hospital setting showed little variability in spaA types, whereas the MRSA clones circulating for a longer period of time and spread among several hospitals showed a higher degree of variability. The implementation of the spaA typing method was straightforward, and the results obtained were reproducible, unambiguous, and easily interpreted. This method seems to be adequate for outbreak investigations but should be complemented with other techniques in long-term surveillance or in studies comparing distant clonal lineages.

* Corresponding author. Mailing address: The Rockefeller University, 1230 York Ave., New York, NY 10021. Phone: (212) 327-8278. Fax: (212) 327-8688. E-mail: lencash{at}mail.rockefeller.edu.

Journal of Clinical Microbiology, February 2001, p. 574-580, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.574-580.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Synnott, A. J., Kuang, Y., Kurimoto, M., Yamamichi, K., Iwano, H., Tanji, Y. (2009). Isolation from Sewage Influent and Characterization of Novel Staphylococcus aureus Bacteriophages with Wide Host Ranges and Potent Lytic Capabilities. Appl. Environ. Microbiol. 75: 4483-4490 [Abstract] [Full Text]  
  • Conceicao, T., Aires de Sousa, M., de Lencastre, H. (2009). Staphylococcal Interspersed Repeat Unit Typing of Staphylococcus aureus: Evaluation of a New Multilocus Variable-Number Tandem-Repeat Analysis Typing Method. J. Clin. Microbiol. 47: 1300-1308 [Abstract] [Full Text]  
  • Moodley, A., Stegger, M., Bagcigil, A. F., Baptiste, K. E., Loeffler, A., Lloyd, D. H., Williams, N. J., Leonard, N., Abbott, Y., Skov, R., Guardabassi, L. (2006). spa typing of methicillin-resistant Staphylococcus aureus isolated from domestic animals and veterinary staff in the UK and Ireland. J Antimicrob Chemother 58: 1118-1123 [Abstract] [Full Text]  
  • Jury, F., Al-Mahrous, M., Apostolou, M., Sandiford, S., Fox, A., Ollier, W., Upton, M. (2006). Rapid cost-effective subtyping of meticillin-resistant Staphylococcus aureus by denaturing HPLC.. J Med Microbiol 55: 1053-1060 [Abstract] [Full Text]  
  • Ruppitsch, W., Indra, A., Stoger, A., Mayer, B., Stadlbauer, S., Wewalka, G., Allerberger, F. (2006). Classifying spa Types in Complexes Improves Interpretation of Typing Results for Methicillin-Resistant Staphylococcus aureus.. J. Clin. Microbiol. 44: 2442-2448 [Abstract] [Full Text]  
  • Soo Ko, K., Kim, Y.-S., Song, J.-H., Yeom, J.-S., Lee, H., Jung, S.-I., Jeong, D.-R., Kim, S.-W., Chang, H.-H., Ki, H. K., Moon, C., Oh, W. S., Peck, K. R., Lee, N. Y. (2005). Genotypic Diversity of Methicillin-Resistant Staphylococcus aureus Isolates in Korean Hospitals. Antimicrob. Agents Chemother. 49: 3583-3585 [Abstract] [Full Text]  
  • Cha, H. Y., Moon, D. C., Choi, C. H., Oh, J. Y., Jeong, Y. S., Lee, Y. C., Seol, S. Y., Cho, D. T., Chang, H.-H., Kim, S.-W., Lee, J. C. (2005). Prevalence of the ST239 Clone of Methicillin-Resistant Staphylococcus aureus and Differences in Antimicrobial Susceptibilities of ST239 and ST5 Clones Identified in a Korean Hospital. J. Clin. Microbiol. 43: 3610-3614 [Abstract] [Full Text]  
  • Gomes, A. R., Vinga, S., Zavolan, M., de Lencastre, H. (2005). Analysis of the Genetic Variability of Virulence-Related Loci in Epidemic Clones of Methicillin-Resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 49: 366-379 [Abstract] [Full Text]  
  • Chung, M., Dickinson, G., de Lencastre, H., Tomasz, A. (2004). International Clones of Methicillin-Resistant Staphylococcus aureus in Two Hospitals in Miami, Florida. J. Clin. Microbiol. 42: 542-547 [Abstract] [Full Text]  
  • Melter, O., Aires de Sousa, M., Urbaskova, P., Jakubu, V., Zemlickova, H., de Lencastre, H. (2003). Update on the Major Clonal Types of Methicillin-Resistant Staphylococcus aureus in the Czech Republic. J. Clin. Microbiol. 41: 4998-5005 [Abstract] [Full Text]  
  • Aires de Sousa, M., de Lencastre, H. (2003). Evolution of Sporadic Isolates of Methicillin-Resistant Staphylococcus aureus (MRSA) in Hospitals and Their Similarities to Isolates of Community-Acquired MRSA. J. Clin. Microbiol. 41: 3806-3815 [Abstract] [Full Text]  
  • Aires de Sousa, M., Bartzavali, C., Spiliopoulou, I., Santos Sanches, I., Crisostomo, M. I., de Lencastre, H. (2003). Two International Methicillin-Resistant Staphylococcus aureus Clones Endemic in a University Hospital in Patras, Greece. J. Clin. Microbiol. 41: 2027-2032 [Abstract] [Full Text]  
  • Aires de Sousa, M., Crisostomo, M. I., Santos Sanches, I., Wu, J. S., Fuzhong, J., Tomasz, A., de Lencastre, H. (2003). Frequent Recovery of a Single Clonal Type of Multidrug-Resistant Staphylococcus aureus from Patients in Two Hospitals in Taiwan and China. J. Clin. Microbiol. 41: 159-163 [Abstract] [Full Text]  
  • Oliveira, D. C., Lencastre, H. d. (2002). Multiplex PCR Strategy for Rapid Identification of Structural Types and Variants of the mec Element in Methicillin-Resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 46: 2155-2161 [Abstract] [Full Text]  
  • Nallapareddy, S. R., Duh, R.-W., Singh, K. V., Murray, B. E. (2002). Molecular Typing of Selected Enterococcus faecalis Isolates: Pilot Study Using Multilocus Sequence Typing and Pulsed-Field Gel Electrophoresis. J. Clin. Microbiol. 40: 868-876 [Abstract] [Full Text]  
  • Crisostomo, M. I., Westh, H., Tomasz, A., Chung, M., Oliveira, D. C., de Lencastre, H. (2001). The evolution of methicillin resistance in Staphylococcus aureus: Similarity of genetic backgrounds in historically early methicillin-susceptible and -resistant isolates and contemporary epidemic clones. Proc. Natl. Acad. Sci. USA 10.1073/pnas.161272898v1 [Abstract] [Full Text]  
  • Aires de Sousa, M., Miragaia, M., Santos Sanches, I., Avila, S., Adamson, I., Casagrande, S. T., Brandileone, M. C. C., Palacio, R., Dell'Acqua, L., Hortal, M., Camou, T., Rossi, A., Velazquez-Meza, M. E., Echaniz-Aviles, G., Solorzano-Santos, F., Heitmann, I., de Lencastre, H. (2001). Three-Year Assessment of Methicillin-Resistant Staphylococcus aureus Clones in Latin America from 1996 to 1998. J. Clin. Microbiol. 39: 2197-2205 [Abstract] [Full Text]  
  • Crisostomo, M. I., Westh, H., Tomasz, A., Chung, M., Oliveira, D. C., de Lencastre, H. (2001). The evolution of methicillin resistance in Staphylococcus aureus: Similarity of genetic backgrounds in historically early methicillin-susceptible and -resistant isolates and contemporary epidemic clones. Proc. Natl. Acad. Sci. USA 98: 9865-9870 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»