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Journal of Clinical Microbiology, February 2001, p. 586-590, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.586-590.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Unusual Occurrence of M Type 77, Antibiotic-Resistant Group A Streptococci in Southern Sweden

A. Jasir,1 A. Tanna,2 A. Efstratiou,2 and C. Schalén1,*

Departments of Infectious Diseases and Medical Microbiology, University of Lund, Lund, Sweden,1 and Respiratory and Systemic Infection Laboratory, Central Public Health Laboratory, London, United Kingdom2

Received 18 July 2000/Returned for modification 8 October 2000/Accepted 26 November 2000

For many years group A streptococci of T type 28 (T28) have been common in southern Sweden; however, since 1995 resistance to both macrolide-lincosamide-streptogramin B (MLS) antibiotics and tetracycline was observed among T28 isolates, which prompted the present studies on clonal relatedness of antibiotic-resistant T28 strains. By extended T typing, 95 of 100 examined tetracycline-resistant strains showed the combination T9-T13-T28; of these, 94 belonged to M type 77 (M77) and one belonged to M73. Three strains were T28-M28 and two were T28-M nontypeable. The serological M77 was confirmed by PCR capture enzyme-linked immunosorbent assay, emm amplicon restriction profiling, and emm sequence typing. Fifty strains were examined for superantigen genes: speA was detected in three blood isolates only, whereas all isolates harbored speB, and only two of the strains were negative for speC. Eighty-nine of the 100 strains were also macrolide resistant, of which 59 were inducibly MLS resistant (IR) and 21 were constitutively MLS resistant (CR), 6 were noninducibly resistant (NI), and 3 had novel subphenotypes recently reported by our group. Resistance genes were determined by PCR and hybridization methods. Eighty-four of the 100 strains harbored tetM. ermB was detected in all CR and IR strains, and mefA was found in all NI strains; both ermB and mefA were identified in two strains with novel subphenotypes. Pulsed-field gel electrophoresis showed that these antibiotic-resistant M77 strains belonged to at least five different clones.

* Corresponding author. Mailing address: Department of Infectious Diseases and Medical Microbiology, Sölvegatan 23, 22362 Lund, Sweden. Phone: 46-46173284. Fax: 46-46135936. E-mail: claes.schalen{at}mmb.lu.se.

Journal of Clinical Microbiology, February 2001, p. 586-590, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.586-590.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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