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Journal of Clinical Microbiology, February 2001, p. 618-621, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.618-621.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Molecular and Phenotypic Characterization of Potentially New Shigella dysenteriae Serotype

Roney S. Coimbra,1 Pascal Lenormand,1 Francine Grimont,1 Philippe Bouvet,1 Shigeru Matsushita,2 and Patrick A. D. Grimont1,*

Unité des Entérobactéries, INSERM U389, Institut Pasteur, Paris, France,1 and Department of Microbiology, Tokyo Metropolitan Research Laboratory of Public Health, Tokyo, Japan2

Received 7 July 2000/Returned for modification 28 August 2000/Accepted 4 November 2000

From September 1997 to November 1998, the French National Center for Salmonella and Shigella received 22 Shigella isolates recovered from 22 different patients suffering from dysentery. None of these isolates reacted with any of the antisera used to identify established Shigella serotypes, but all of them agglutinated in the presence of antisera to a previously described potentially new Shigella dysenteriae serotype (represented by strain 96-204) primarily isolated from stool cultures of imported diarrheal cases in Japan. All French isolates, as well as strain 96-204, showed biochemical reactions typical of S. dysenteriae and gave positive results in a PCR assay for detection of the plasmid ipaH gene coding for invasiveness. No Shiga toxin gene was detected by PCR. These isolates were indistinguishable by molecular analysis of ribosomal DNA (ribotyping) and seemed to be related to S. dysenteriae serotypes 3 and 12. However, further characterization by restriction of the amplified O-antigen gene cluster clearly distinguished this new serotype from all other Shigella or Escherichia coli serotypes.

* Corresponding author. Mailing address: Unité des Entérobactéries, Institut Pasteur, 28, Rue du Docteur Roux, 75724 Paris Cedex 15, France. Phone: 33 145688340. Fax: 33 145688837. E-mail: pgrimont{at}pasteur.fr.

Journal of Clinical Microbiology, February 2001, p. 618-621, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.618-621.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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