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Journal of Clinical Microbiology, February 2001, p. 658-669, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.658-669.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Cloning and Characterization of a Complex DNA Fingerprinting Probe for Candida parapsilosis

Lee Enger,1 Sophie Joly,1 Claude Pujol,1 Patricia Simonson,1 Michael Pfaller,2 and David R. Soll1,*

Department of Biological Sciences1 and Pathology,2 University of Iowa, Iowa City, Iowa 52242

Received 5 September 2000/Returned for modification 13 November 2000/Accepted 29 November 2000

Candida parapsilosis accounts for a significant number of nosocomial fungemias, but in fact, no effective and verified genetic fingerprinting method has emerged for assessing the relatedness of independent isolates for epidemiological studies. A complex 15-kb DNA fingerprinting probe, Cp3-13, was therefore isolated from a library of C. parapsilosis genomic DNA fragments. The efficacy of Cp3-13 for DNA fingerprinting was verified by a comparison of its clustering capacity with those of randomly amplified polymorphic DNA analysis and internally transcribed spacer region sequencing, by testing species specificity, and by assessing its capacity to identify microevolutionary changes both in vitro and in vivo. Southern blot hybridization of EcoRI/SalI-digested DNA with Cp3-13 provides a fingerprinting system that (i) identifies the same strain in independent isolates, (ii) discriminates between unrelated isolates, (iii) separates independent isolates into valid groups in a dendrogram, (iv) identifies microevolution in infecting populations, and (v) is amenable to automatic computer-assisted DNA fingerprint analysis. This probe is now available for epidemiological studies.

* Corresponding author. Mailing address: Room 302 BBE, Department of Biological Sciences, University of Iowa, Iowa City, IA 52242. Phone: (319) 335-1117. Fax: (319) 335-2772. E-mail: david-soll{at}uiowa.edu.

Journal of Clinical Microbiology, February 2001, p. 658-669, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.658-669.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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