Evaluation of a Rapid Optical Immunoassay for Influenza Viruses (FLU OIA Test) in Comparison with Cell Culture and Reverse Transcription-PCR

doi:10.1128/JCM.39.2.730-732.2001

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Journal of Clinical Microbiology, February 2001, p. 730-732, Vol. 39, No. 2
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.2.730-732.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Evaluation of a Rapid Optical Immunoassay for Influenza Viruses (FLU OIA Test) in Comparison with Cell Culture and Reverse Transcription-PCR

Guy Boivin,¹^,^* Isabelle Hardy,¹ and Andrew Kress²

Research Center in Infectious Diseases of the Centre Hospitalier Universitaire de Québec and Laval University, Québec City, Quebec, Canada,¹ and Surveillance Data, Inc., Philadelphia, Pennsylvania²

Received 13 July 2000/Returned for modification 8 September 2000/Accepted 8 November 2000

The FLU OIA test was evaluated with 146 throat swab specimens from subjects with a flu-like illness in six Canadian clinicsduring the 1999-2000 flu season. The rate of positivity of theFLU OIA test (41.5%) was significantly lower than that of cellculture (55.2%) or reverse transcription-PCR (55.9%) during aseason in which only influenza A virus wasdetected.

^* Corresponding author. Mailing address: CHUQ, pavillon CHUL, room RC-709, 2705 blvd. Laurier, Sainte-Foy, Québec, Canada GIV4G2. Phone: (418) 654-2705. Fax: (418) 654-2715. E-mail: Guy.Boivin{at}crchul.ulaval.ca.

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