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Journal of Clinical Microbiology, February 2001, p. 750-753, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.750-753.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Protocol for Detection of Biofilms on Needleless Connectors Attached to Central Venous Catheters

R. M. Donlan,1,* R. Murga,1 M. Bell,1 C. M. Toscano,1 J. H. Carr,1 T. J. Novicki,2 C. Zuckerman,2 L. C. Corey,2 and J. M. Miller1

Hospital Infections Program, Centers for Disease Control and Prevention, Atlanta, Georgia 30333,1 and Fred Hutchinson Cancer Research Center, Seattle, Washington 981092

Received 19 July 2000/Returned for modification 8 September 2000/Accepted 13 November 2000

Central venous catheter needleless connectors (NCs) have been shown to develop microbial contamination. A protocol was developed for the collection, processing, and examination of NCs to detect and measure biofilms on these devices. Sixty-three percent of 24 NCs collected from a bone marrow transplant center contained biofilms comprised primarily of coagulase-negative staphylococci.

* Corresponding author. Mailing address: Hospital Infections Program, CDC, Mail Stop C-16, Atlanta, GA 30333. Phone: (404) 639-2322. Fax: (404) 639-3241. E-mail: rld8{at}cdc.gov.

Journal of Clinical Microbiology, February 2001, p. 750-753, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.750-753.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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