This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Marin, I. J.
Right arrow Articles by Brinovec, V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Marin, I. J.
Right arrow Articles by Brinovec, V.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, February 2001, p. 758-761, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.758-761.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Comparative Evaluation of Semiautomated COBAS AMPLICOR Hepatitis B Virus (HBV) MONITOR Test and Manual Microwell Plate-Based AMPLICOR HBV MONITOR Test

Irena J. Marin,1 Mario Poljak,1,* Katja Seme,1 Jelka Meglic-Volkar,2 Mojca Maticic,2 Gorazd Lesnicar,3 and Vladimir Brinovec2

Institute of Microbiology and Immunology, Medical Faculty,1 and Department of Infection Diseases, Clinical Center,2 Ljubljana, and Department of Infection Diseases, General Hospital, Celje,3 Slovenia

Received 8 August 2000/Accepted 4 December 2000

Comparative evaluation of the semiautomated COBAS AMPLICOR hepatitis B virus (HBV) MONITOR Test (COBAS-HBV) and manual AMPLICOR HBV MONITOR Test (AMPLICOR-HBV) on 208 serum samples revealed no significant difference in the sensitivities of the two assays. Twenty samples tested HBV DNA negative and 183 samples tested HBV DNA positive by both assays. Three samples tested positive by COBAS-HBV only and two samples tested positive by AMPLICOR-HBV only. HBV DNA concentrations determined by the two assays were significantly related (n = 183, r = 0.97, P < 0.0001), which indicates that COBAS-HBV could replace AMPLICOR-HBV. The major inconvenience of COBAS-HBV is the required performance of appropriate predilutions of high-titer samples in order to extend the narrow dynamic range of the assay.

* Corresponding author. Mailing address: Institute of Microbiology and Immunology, Medical Faculty, Zaloska 4, 1105 Ljubljana, Slovenia. Phone: 386 1 543 7453. Fax: 386 1 543 7401. E-mail: mario.poljak{at}mf.uni-lj.si.

Journal of Clinical Microbiology, February 2001, p. 758-761, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.758-761.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Laperche, S., Thibault, V., Bouchardeau, F., Alain, S., Castelain, S., Gassin, M., Gueudin, M., Halfon, P., Larrat, S., Lunel, F., Martinot-Peignoux, M., Mercier, B., Pawlotsky, J.-M., Pozzetto, B., Roque-Afonso, A.-M., Roudot-Thoraval, F., Saune, K., Lefrere, J.-J. (2006). Expertise of laboratories in viral load quantification, genotyping, and precore mutant determination for hepatitis B virus in a multicenter study.. J. Clin. Microbiol. 44: 3600-3607 [Abstract] [Full Text]  
  • Welzel, T. M., Miley, W. J., Parks, T. L., Goedert, J. J., Whitby, D., Ortiz-Conde, B. A. (2006). Real-time PCR assay for detection and quantification of hepatitis B virus genotypes a to g.. J. Clin. Microbiol. 44: 3325-3333 [Abstract] [Full Text]  
  • Hui, C.-K., Bowden, S., Zhang, H.-Y., Wong, A., Lewin, S., Rousseau, F., Mommeja-Marin, H., Lee, N. P., Luk, J. M., Locarnini, S., Leung, N., Naoumov, N. V., Lau, G. K. K. (2006). Comparison of Real-Time PCR Assays for Monitoring Serum Hepatitis B Virus DNA Levels during Antiviral Therapy.. J. Clin. Microbiol. 44: 2983-2987 [Abstract] [Full Text]  
  • Gordillo, R. M., Gutierrez, J., Casal, M. (2005). Evaluation of the COBAS TaqMan 48 Real-Time PCR System for Quantitation of Hepatitis B Virus DNA. J. Clin. Microbiol. 43: 3504-3507 [Abstract] [Full Text]  
  • Konnick, E. Q., Erali, M., Ashwood, E. R., Hillyard, D. R. (2005). Evaluation of the COBAS Amplicor HBV Monitor Assay and Comparison with the Ultrasensitive HBV Hybrid Capture 2 Assay for Quantification of Hepatitis B Virus DNA. J. Clin. Microbiol. 43: 596-603 [Abstract] [Full Text]  
  • Stelzl, E., Muller, Z., Marth, E., Kessler, H. H. (2004). Rapid Quantification of Hepatitis B Virus DNA by Automated Sample Preparation and Real-Time PCR. J. Clin. Microbiol. 42: 2445-2449 [Abstract] [Full Text]  
  • Valentine-Thon, E., van Loon, A. M., Schirm, J., Reid, J., Klapper, P. E., Cleator, G. M. (2001). European Proficiency Testing Program for Molecular Detection and Quantitation of Hepatitis B Virus DNA. J. Clin. Microbiol. 39: 4407-4412 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»