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Journal of Clinical Microbiology, February 2001, p. 765-768, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.765-768.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Quantitative Detection of Hepatitis C Virus RNA by Light Cycler PCR and Comparison with Two Different PCR Assays

Matthias Schröter,* Bernhard Zöllner, Peter Schäfer, Rainer Laufs, and Heinz-Hubert Feucht

Institut für Medizinische Mikrobiologie und Immunologie, Universitätsklinikum Hamburg-Eppendorf, 20246 Hamburg, Germany

Received 28 June 2000/Accepted 21 November 2000

The new Light Cycler technology was adapted to the detection of hepatitis C virus (HCV) RNA in clinical samples. Sera from 81 patients were tested by Light Cycler PCR, AMPLICOR HCV Monitor assay, and in-house PCR. Our data demonstrate that Light Cycler is a fast and reliable method for the detection and quantitation of HCV RNA.

* Corresponding author. Mailing address: Institut für Medizinische Mikrobiologie und Immunologie, Universitätsklinikum Hamburg-Eppendorf, Martinistraße 52, 20246 Hamburg, Germany. Phone: 49 (0) 40-42803-3159. Fax: 49 (0) 40-42803-4062. E-mail: mschroet{at}uke.uni-hamburg.de.

Journal of Clinical Microbiology, February 2001, p. 765-768, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.765-768.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
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Copyright © 2001 by the American Society for Microbiology. All rights reserved.