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Journal of Clinical Microbiology, February 2001, p. 772-775, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.772-775.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Quantification of Human Cytomegalovirus DNA by Real-Time PCR

Elyanne Gault,1,2,dagger Yanne Michel,1 Axelle Dehée,1,2 Chahrazed Belabani,1 Jean-Claude Nicolas,2,3 and Antoine Garbarg-Chenon1,2,*

Laboratoire de Virologie, Hôpital Armand Trousseau,1 Laboratoire de Virologie, Hôpital Rothschild,3 and EA 2391, UFR Saint-Antoine,2 Paris, France

Received 28 September 2000/Returned for modification 23 October 2000/Accepted 27 November 2000

A quantitative real-time PCR assay was developed to measure human cytomegalovirus (HCMV) DNA load in peripheral blood leukocytes (PBLs). The HCMV DNA load in PBLs was normalized by means of the quantification of a cellular gene (albumin). The results of the real-time PCR assay correlated with those of the HCMV pp65-antigenemia assay (P < 0.0001).

* Corresponding author. Mailing address: Laboratoire de Virologie, Hôpital Armand Trousseau, 26 Avenue du Dr. Arnold Netter 75571, Paris Cedex 12, France. Phone: 33 1 44 73 62 81. Fax: 33 1 44 73 62 88. E-mail: a.chenon{at}trs.ap-hop-paris.fr.

dagger Present address: Laboratoire de Virologie, Hôpital Avicenne, 93009 Bobigny cedex.

Journal of Clinical Microbiology, February 2001, p. 772-775, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.772-775.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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