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Journal of Clinical Microbiology, February 2001, p. 776-778, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.776-778.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Immunofluorescence Method for Detection of Ebola Virus Immunoglobulin G, Using HeLa Cells Which Express Recombinant Nucleoprotein

Masayuki Saijo, Masahiro Niikura, Shigeru Morikawa,* and Ichiro Kurane

Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Gakuen 4-7-1, Musahimurayama, Tokyo 208-0011, Japan

Received 21 August 2000/Returned for modification 29 September 2000/Accepted 15 November 2000

A novel recombinant baculovirus which expresses Ebola virus (EBO) nucleoprotein (NP) under the control of the cytomegalovirus immediate-early promoter was constructed. HeLa cells abortively infected with the baculovirus expressed EBO NP, and this was used as an immunofluorescent (IF) antigen to detect EBO immunoglobulin G (IgG) antibody. This IF method has high efficacy in detecting EBO IgG antibody in clinical specimens, indicating its usefulness in the diagnosis of EBO infections and seroepidemiological studies.

* Corresponding author. Mailing address: Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Gakuen 4-7-1, Musahimurayama, Tokyo 208-0011, Japan. Phone: 81-42-561-0771 (791). Fax: 81-42-564-4881. E-mail: morikawa{at}nih.go.jp.

Journal of Clinical Microbiology, February 2001, p. 776-778, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.776-778.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Saijo, M., Niikura, M., Ikegami, T., Kurane, I., Kurata, T., Morikawa, S. (2006). Laboratory Diagnostic Systems for Ebola and Marburg Hemorrhagic Fevers Developed with Recombinant Proteins. CVI 13: 444-451 [Full Text]  
  • Ikegami, T., Niikura, M., Saijo, M., Miranda, M. E., Calaor, A. B., Hernandez, M., Acosta, L. P., Manalo, D. L., Kurane, I., Yoshikawa, Y., Morikawa, S. (2003). Antigen Capture Enzyme-Linked Immunosorbent Assay for Specific Detection of Reston Ebola Virus Nucleoprotein. CVI 10: 552-557 [Abstract] [Full Text]  
  • Niikura, M., Ikegami, T., Saijo, M., Kurata, T., Kurane, I., Morikawa, S. (2003). Analysis of Linear B-Cell Epitopes of the Nucleoprotein of Ebola Virus That Distinguish Ebola Virus Subtypes. CVI 10: 83-87 [Abstract] [Full Text]  
  • Niikura, M., Ikegami, T., Saijo, M., Kurane, I., Miranda, M. E., Morikawa, S. (2001). Detection of Ebola Viral Antigen by Enzyme-Linked Immunosorbent Assay Using a Novel Monoclonal Antibody to Nucleoprotein. J. Clin. Microbiol. 39: 3267-3271 [Abstract] [Full Text]  


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