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Journal of Clinical Microbiology, February 2001, p. 794-797, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.794-797.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Routine Molecular Identification of Enterococci by Gene-Specific PCR and 16S Ribosomal DNA Sequencing

Silvia Angeletti,1 Giulia Lorino,2 Giovanni Gherardi,1 Fabrizio Battistoni,1 Marina De Cesaris,1 and Giordano Dicuonzo1,*

Libera Università Campus Bio-Medico, 00155 Rome,1 and Istituto Microbiologia, Facoltà Medicina e Chirurgia, Università "La Sapienza", 00187 Rome,2 Italy

Received 21 August 2000/Returned for modification 16 October 2000/Accepted 4 December 2000

For 279 clinically isolated specimens identified by commercial kits as enterococci, genotypic identification was performed by two multiplex PCRs, one with ddlE. faecalis and ddlE. faecium primers and another with vanC-1 and vanC-2/3 primers, and by 16S ribosomal DNA (rDNA) sequencing. For 253 strains, phenotypic and genotypic results were the same. Multiplex PCR allowed for the identification of 13 discordant results. Six strains were not enterococci and were identified by 16S rDNA sequencing. For 5 discordant and 10 concordant enterococcal strains, 16S rDNA sequencing was needed. Because many supplementary tests are frequently necessary for phenotypic identification, the molecular approach is a good alternative.

* Corresponding author. Mailing address: Libera Università Campus Bio-Medico, Via E. Longoni 83, 00155 Rome, Italy. Phone: 39-0622541790. Fax: 39-0622541456. E-mail: g.dicuonzo{at}unicampus.it.

Journal of Clinical Microbiology, February 2001, p. 794-797, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.794-797.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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