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Journal of Clinical Microbiology, March 2001, p. 1021-1024, Vol. 39, No. 3
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.3.1021-1024.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Evaluation of a Medium (STGG) for Transport and Optimal Recovery
of Streptococcus pneumoniae from Nasopharyngeal
Secretions Collected during Field Studies
Katherine L.
O'Brien,1,2,*
Melinda
A.
Bronsdon,1
Ron
Dagan,3,4
Pablo
Yagupsky,3,4
Jacob
Janco,3,4
John
Elliott,1
Cynthia G.
Whitney,1
Yong-Hong
Yang,5
Lisa-Gaye E.
Robinson,6
Benjamin
Schwartz,1 and
George
M.
Carlone1
Respiratory Diseases Branch, Division of Bacterial and
Mycotic Diseases,1 and Epidemic
Intelligence Service, Epidemiology Program
Office,2 Centers for Disease Control and
Prevention, and Department of Pediatrics, Egleston
Children's Hospital, Emory University,6
Atlanta, Georgia; Soroka University Medical
Center3 and Faculty of Health
Sciences,4 Ben Gurion University, Beer
Sheva, Israel; and Beijing Children's Hospital, Beijing,
People's Republic of China5
Received 10 October 2000/Returned for modification 20 November
2000/Accepted 22 December 2000
Field studies of Streptococcus pneumoniae
(pneumococci) nasopharyngeal (NP) colonization are hampered by the need
to directly plate specimens in order to ensure isolate viability. A
medium containing skim milk, tryptone, glucose, and glycerin (STGG) has been used to transport and store NP material, but its ability to
preserve pneumococci has not been evaluated. Our objective was to
qualitatively and semiquantitatively evaluate the ability of STGG to
preserve pneumococci in NP secretions. Entwined duplicate calcium
alginate NP swab samples were obtained from children. One swab was
plated directly onto a gentamicin blood agar plate; the other was
placed in STGG. Growth from the directly plated specimen was compared
with growth from an STGG aliquot immediately cultured or stored at
70°C for 9 weeks,
20°C for 9 weeks, or 4°C for 5 days. Of 186 specimens, 96 (52%) were positive for pneumococci from the direct
plating; 94 (98%) of these were positive from the fresh STGG specimen.
Pneumococci were recovered from all 38 positive specimens frozen at
70°C, all 18 positive specimens frozen at
20°C, and 18 of 20 positive specimens stored at 4°C. Recovery of pneumococci after
storage of NP material in STGG medium at
70°C is at least as good
as that from direct plating. Storage at
20°C is also acceptable.
Storage at 4°C for 5 days is not ideal.
*
Corresponding author. Present address: Center for
American Indian and Alaskan Native Health, Johns Hopkins School of
Hygiene and Public Health, 621 N. Washington St., Baltimore, MD
21205. Phone: (410) 614-3806. Fax: (410) 955-2010. E-mail:
klobrien{at}jhsph.edu.
Journal of Clinical Microbiology, March 2001, p. 1021-1024, Vol. 39, No. 3
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.3.1021-1024.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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