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Journal of Clinical Microbiology, March 2001, p. 1079-1084, Vol. 39, No. 3
Centro Regionale di Riferimento per la
Diagnostica delle Micobatteriosi, Laboratorio di Microbiologia e
Virologia, Ospedale di Careggi, Firenze,1
Istituto di Microbiologia, Università di Bologna,
Bologna,2 Dipartimento di Microbiologia,
Ospedale Umberto I-Torrette, Ancona,3
Laboratorio di Microbiologia, Ospedale S. Raffaele,
Milano,4 Servizio di Microbiologia,
Ospedali Riuniti, Bergamo,5 U.O. di
Microbiologia e Immunologia, Ospedale S. Maria degli Angeli,
Pordenone,6 and Laboratorio di
Microbiologia, Ospedale S. Bortolo, Vicenza,7
Italy
Received 25 September 2000/Returned for modification 14 November
2000/Accepted 7 December 2000
A new DNA probe assay (INNO LiPA Mycobacteria; Innogenetics, Ghent,
Belgium) for the simultaneous identification, by means of reverse
hybridization and line-probe technology, of Mycobacterium tuberculosis complex, Mycobacterium kansasii,
Mycobacterium xenopi, Mycobacterium gordonae,
the species of the Mycobacterium avium complex (MAC),
Mycobacterium scrofulaceum, and Mycobacterium
chelonae was evaluated on a panel of 238 strains including,
besides representatives of all the taxa identifiable by the system, a
number of other mycobacteria, some of which are known to be problematic
with the only other commercial DNA probe system (AccuProbe; Gen-Probe, San Diego, Calif.), and two nocardiae. The new kit, which includes a
control probe reacting with the whole genus Mycobacterium,
correctly identified 99.6% of the strains tested; the one discrepancy,
which remained unresolved, concerned an isolate identified as MAC
intermediate by INNO LiPA Mycobacteria and as Mycobacterium
intracellulare by AccuProbe. In five cases, because of an
imperfect checking of hybridization temperature, a very slight,
nonspecific, line was visible which was no longer evident when the test
was repeated. Two strains whose DNA failed amplification at the first
attempt were regularly identified when the test was repeated.
Interestingly, the novel kit dodged all the pitfalls presented by the
strains giving anomalous reactions with AccuProbe. A unique feature of INNO LiPA Mycobacteria is its ability to recognize different subgroups within the species M. kansasii and M. chelonae,
while the declared overlapping reactivity of probe 4 with some M. kansasii and Mycobacterium gastri organisms and of
probe 9 with MAC, Mycobacterium haemophilum, and
Mycobacterium malmoense, may furnish a useful aid for their identification. The turnaround time of the method is approximately 6 h, including a preliminary PCR amplification.
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.3.1079-1084.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Performance Assessment of New Multiplex Probe Assay
for Identification of Mycobacteria
*
Corresponding author. Mailing address: Laboratorio di
Microbiologia e Virologia, Ospedale di Careggi, Piastra dei servizi, viale Morgagni 85, 50134 Firenze, Italy. Phone: 39-055-4279199. Fax:
39-055-4279830. E-mail: tortoli{at}dada.it.
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