This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Westin, L.
Right arrow Articles by O'Connell, J. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Westin, L.
Right arrow Articles by O'Connell, J. P.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, March 2001, p. 1097-1104, Vol. 39, No. 3
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.3.1097-1104.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Antimicrobial Resistance and Bacterial Identification Utilizing a Microelectronic Chip Array

Lorelei Westin,1 Carolyn Miller,2,* Dana Vollmer,2 David Canter,3 Ray Radtkey,3 Michael Nerenberg,3,dagger and James P. O'Connell1

Departments of Advanced Research,1 Assay Development,2 and Molecular Biology,3 Nanogen, Inc., San Diego, California

Received 31 July 2000/Returned for modification 19 September 2000/Accepted 8 December 2000

Species-specific bacterial identification of clinical specimens is often limited to a few species due to the difficulty of performing multiplex reactions. In addition, discrimination of amplicons is time-consuming and laborious, consisting of gel electrophoresis, probe hybridization, or sequencing technology. In order to simplify the process of bacterial identification, we combined anchored in situ amplification on a microelectronic chip array with discrimination and detection on the same platform. Here, we describe the simultaneous amplification and discrimination of six gene sequences which are representative of different bacterial identification assays: Escherichia coli gyrA, Salmonella gyrA, Campylobacter gyrA, E. coli parC, Staphylococcus mecA, and Chlamydia cryptic plasmid. The assay can detect both plasmid and transposon genes and can also discriminate strains carrying antibiotic resistance single-nucleotide polymorphism mutations. Finally, the assay is similarly capable of discriminating between bacterial species through reporter-specific discrimination and allele-specific amplification. Anchored strand displacement amplification allows multiplex amplification and complex genotype discrimination on the same platform. This assay simplifies the bacterial identification process greatly, allowing molecular biology techniques to be performed with minimal processing of samples and practical experience.

* Corresponding author. Mailing address: Assay Development, Nanogen, Inc., 10398 Pacific Center Ct., San Diego, CA 92121. Phone: (858) 410-4718. Fax: (858) 410-4848. E-mail: Cmiller{at}nanogen.com.

dagger Present address: Molecular Reflections, San Diego, Calif.

Journal of Clinical Microbiology, March 2001, p. 1097-1104, Vol. 39, No. 3
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.3.1097-1104.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Sanguinetti, M., Novarese, L., Posteraro, B., Ranno, S., De Carolis, E., Pecorini, G., Lucignano, B., Ardito, F., Fadda, G. (2005). Use of Microelectronic Array Technology for Rapid Identification of Clinically Relevant Mycobacteria. J. Clin. Microbiol. 43: 6189-6193 [Abstract] [Full Text]  
  • Lim, D. V., Simpson, J. M., Kearns, E. A., Kramer, M. F. (2005). Current and Developing Technologies for Monitoring Agents of Bioterrorism and Biowarfare. Clin. Microbiol. Rev. 18: 583-607 [Abstract] [Full Text]  
  • Peytavi, R., Raymond, F. R., Gagne, D., Picard, F. J., Jia, G., Zoval, J., Madou, M., Boissinot, K., Boissinot, M., Bissonnette, L., Ouellette, M., Bergeron, M. G. (2005). Microfluidic Device for Rapid (<15 min) Automated Microarray Hybridization. Clin. Chem. 51: 1836-1844 [Abstract] [Full Text]  
  • Saunders, N. A., Alexander, S., Tatt, I. (2005). env Gene Typing of Human Immunodeficiency Virus Type 1 Strains on Electronic Microarrays. J. Clin. Microbiol. 43: 1910-1916 [Abstract] [Full Text]  
  • Xing, J. Z., Clarke, C., Zhu, L., Gabos, S. (2005). Development of a Microelectronic Chip Array for High-Throughput Genotyping of Helicobacter Species and Screening for Antimicrobial Resistance. J Biomol Screen 10: 235-245 [Abstract]  
  • Santacroce, R., Ratti, A., Caroli, F., Foglieni, B., Ferraris, A., Cremonesi, L., Margaglione, M., Seri, M., Ravazzolo, R., Restagno, G., Dallapiccola, B., Rappaport, E., Pollak, E. S., Surrey, S., Ferrari, M., Fortina, P. (2002). Analysis of Clinically Relevant Single-Nucleotide Polymorphisms by Use of Microelectronic Array Technology. Clin. Chem. 48: 2124-2130 [Abstract] [Full Text]  
  • Hakanen, A., Jalava, J., Kotilainen, P., Jousimies-Somer, H., Siitonen, A., Huovinen, P. (2002). gyrA Polymorphism in Campylobacter jejuni: Detection of gyrA Mutations in 162 C. jejuni Isolates by Single-Strand Conformation Polymorphism and DNA Sequencing. Antimicrob. Agents Chemother. 46: 2644-2647 [Abstract] [Full Text]  
  • Garaizar, J., Porwollik, S., Echeita, A., Rementeria, A., Herrera, S., Wong, R. M.-Y., Frye, J., Usera, M. A., McClelland, M. (2002). DNA Microarray-Based Typing of an Atypical Monophasic Salmonella enterica Serovar. J. Clin. Microbiol. 40: 2074-2078 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»