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Journal of Clinical Microbiology, March 2001, p. 1118-1120, Vol. 39, No. 3
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.3.1118-1120.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Effect of Errors in the Sequence of Optical Densities from the Roche AMPLICOR HIV-1 MONITOR Assay on the Validity of Assay Results

Donald J. Brambilla,1,* Suzanne Granger,1 Cheryl Jennings,2 and James W. Bremer2

New England Research Institutes, Inc., Watertown, Massachusetts 02472,1 and Rush Medical College, Chicago, Illinois 606122

Received 11 May 2000/Returned for modification 23 October 2000/Accepted 15 December 2000

Specifications for the AMPLICOR HIV-1 MONITOR kit indicate that the results are invalid if the optical densities (ODs) from the PCR-amplified sample that are between 0.1 and 2.3 units are out of sequence. However, among 11,904 assays, results were biased only when ODs of 0.2 to 2.0 units were out of sequence, reducing the rate of invalid results from 3.2 to 0.59%.

* Corresponding author. Mailing address: New England Research Institutes, 9 Galen St., Watertown, MA 02472. Phone: (617) 923-7747, ext. 230. Fax: (617) 926-1142. E-mail: donb{at}neri.org.

Journal of Clinical Microbiology, March 2001, p. 1118-1120, Vol. 39, No. 3
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.3.1118-1120.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 2001 by the American Society for Microbiology. All rights reserved.