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Journal of Clinical Microbiology, March 2001, p. 1130-1133, Vol. 39, No. 3
Department of Medicine, Rheumatology and
Clinical Immunology1 and Department of
Neurology,2 Charité University
Hospital, Berlin, Germany; Department of Dermatology, University of
Graz, Graz, Austria3; and National
Institute of Public Health and the Environment, Bilthoven, The
Netherlands4
Received 5 May 2000/Returned for modification 27 July 2000/Accepted 15 December 2000
To further investigate the pathogenic potential of different
Borrelia burgdorferi genospecies, specimens from 27 patients with different manifestations of Lyme borreliosis were
analyzed by PCR and reverse line blotting (RLB). In samples from Lyme
arthritis patients, B. burgdorferi sensu stricto was
predominantly identified, while in patients with neuroborreliosis or
acrodermatitis, Borrelia garinii and Borrelia
afzelii, respectively, were exclusively detected. The results
demonstrate that PCR-RLB is a valuable tool for epidemiological and
pathogenetic studies of Lyme borreliosis.
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.3.1130-1133.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Rapid Typing of Borrelia burgdorferi
Sensu Lato Species in Specimens from Patients with Different
Manifestations of Lyme Borreliosis
*
Corresponding author. Mailing address: Department of
Medicine, Rheumatology and Clinical Immunology, Charité
University Hospital, 10098 Berlin, Germany. Phone: 49 30 2802 5773. Fax: 49 30 2802 5098. E-mail: andreas.krause{at}charite.de.
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