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Journal of Clinical Microbiology, March 2001, p. 1130-1133, Vol. 39, No. 3
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.3.1130-1133.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Rapid Typing of Borrelia burgdorferi Sensu Lato Species in Specimens from Patients with Different Manifestations of Lyme Borreliosis

Jan D. Lünemann,1 Silvia Zarmas,1 Susanne Priem,1 Juliane Franz,1 Rolf Zschenderlein,2 Elisabeth Aberer,3 Rolf Klein,1 Leo Schouls,4 Gerd R. Burmester,1 and Andreas Krause1,*

Department of Medicine, Rheumatology and Clinical Immunology1 and Department of Neurology,2 Charité University Hospital, Berlin, Germany; Department of Dermatology, University of Graz, Graz, Austria3; and National Institute of Public Health and the Environment, Bilthoven, The Netherlands4

Received 5 May 2000/Returned for modification 27 July 2000/Accepted 15 December 2000

To further investigate the pathogenic potential of different Borrelia burgdorferi genospecies, specimens from 27 patients with different manifestations of Lyme borreliosis were analyzed by PCR and reverse line blotting (RLB). In samples from Lyme arthritis patients, B. burgdorferi sensu stricto was predominantly identified, while in patients with neuroborreliosis or acrodermatitis, Borrelia garinii and Borrelia afzelii, respectively, were exclusively detected. The results demonstrate that PCR-RLB is a valuable tool for epidemiological and pathogenetic studies of Lyme borreliosis.


* Corresponding author. Mailing address: Department of Medicine, Rheumatology and Clinical Immunology, Charité University Hospital, 10098 Berlin, Germany. Phone: 49 30 2802 5773. Fax: 49 30 2802 5098. E-mail: andreas.krause{at}charite.de.


Journal of Clinical Microbiology, March 2001, p. 1130-1133, Vol. 39, No. 3
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.3.1130-1133.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.



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Copyright © 2001 by the American Society for Microbiology. All rights reserved.