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Journal of Clinical Microbiology, March 2001, p. 1155-1156, Vol. 39, No. 3
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.3.1155-1156.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Quantitation of Porcine Cytomegalovirus in Pig Tissues by PCR

Jacqueline F. L. Fryer,1 Paul D. Griffiths,1 Jay A. Fishman,2 Vincent C. Emery,1 and Duncan A. Clark1,*

Department of Virology, Royal Free and University College Medical School of UCL, London, United Kingdom,1 and Transplant Infectious Disease Program, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts2

Received 5 September 2000/Returned for modification 27 November 2000/Accepted 21 December 2000

A quantitative-competitive PCR for the quantification of porcine cytomegalovirus (PCMV) was developed. The virus was detected in a variety of pig organs (including potential xenotransplant donations), with viral loads ranging from <10 to 97 genome copies/µg of DNA. This assay will have significant utility for studying the activation and replication of PCMV and in swine models for allo- and xenotransplantation.

* Corresponding author. Mailing address: Department of Virology, Royal Free and University College Medical School, Royal Free Campus, Rowland Hill Street, London NW3 2PF, United Kingdom. Phone: 44-20-78302997. Fax: 44-20-78302854. E-mail: d.clark{at}rfc.ucl.ac.uk.

Journal of Clinical Microbiology, March 2001, p. 1155-1156, Vol. 39, No. 3
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.3.1155-1156.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Garkavenko, O., Croxson, M. C., Irgang, M., Karlas, A., Denner, J., Elliott, R. B. (2004). Monitoring for Presence of Potentially Xenotic Viruses in Recipients of Pig Islet Xenotransplantation. J. Clin. Microbiol. 42: 5353-5356 [Abstract] [Full Text]  


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