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Journal of Clinical Microbiology, March 2001, p. 1172-1174, Vol. 39, No. 3
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.3.1172-1174.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Rapid Identification of Candida glabrata Based on Trehalose and Sucrose Assimilation Using Rosco Diagnostic Tablets

José Lopez, Frédéric Dalle, Pierre Mantelin, Philippe Moiroux, Anne-Charlotte Nierlich, Agnès Pacot, Bernadette Cuisenier, Odile Vagner, and Alain Bonnin*

Laboratoire de Parasitologie et Mycologie, Hôpital du Bocage, 21034 Dijon Cedex, France

Received 10 July 2000/Returned for modification 16 October 2000/Accepted 20 December 2000

We developed a simple method for the identification of Candida glabrata on the basis of the ability of this species to rapidly assimilate trehalose but not sucrose. After incubation of yeasts with Rosco diagnostic tablets containing sucrose or trehalose, identification of C. glabrata was achieved in 4 h with 100% sensitivity and specificity.

* Corresponding author. Mailing address: Laboratoire de Parasitologie et Mycologie, Hôpital du Bocage, 21034 Dijon Cedex, France. Phone: 33 380 29 36 03. Fax: 33 380 29 32 80. E-mail: alain.bonnin{at}chu-dijon.fr.

Journal of Clinical Microbiology, March 2001, p. 1172-1174, Vol. 39, No. 3
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.3.1172-1174.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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