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Journal of Clinical Microbiology, March 2001, p. 959-963, Vol. 39, No. 3
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.3.959-963.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Use of Immunoglobulin G Antibody Avidity for Differentiation of Primary Human Herpesvirus 6 and 7 Infections

K. N. Ward,1,2,* D. J. Turner,1,dagger X. Couto Parada,2 and A. D. Thiruchelvam2

Department of Infectious Diseases, Imperial College School of Medicine, Hammersmith Campus, London W12 ONN,1 and Department of Virology, Royal Free and University College Medical School, London W1T 4JF,2 United Kingdom

Received 14 August 2000/Returned for modification 6 November 2000/Accepted 22 December 2000

A human herpesvirus 7 (HHV-7) indirect immunofluorescence antibody avidity test was developed and used with an existing human herpesvirus 6 (HHV-6) antibody avidity test to detect and distinguish low-avidity antibodies to HHV-6 and HHV-7 and hence the respective primary infections. With sera from 269 British children aged 0 to 179 weeks, the tests showed that most (10 of 98 serum samples [13%]) HHV-6 low-avidity antibody was found in the first year of life, whereas for HHV-7, most (18 of 101 serum samples [20%]) HHV-7 low-avidity antibody was found in the second year of life. Five children had low-avidity antibodies to both viruses. Of nine Japanese children with previously serologically proven primary HHV-6 or HHV-7 infections, eight had low-avidity antibody only to the relevant virus, but one child had low-avidity antibodies to HHV-6 and HHV-7. The avidity tests were applied to five British children and further proof of viral infection was sought by the detection of specific DNA in serum or plasma, and saliva or cerebrospinal fluid. In two children who had low-avidity antibody to HHV-7 but who were seronegative for HHV-6, only HHV-7 was found. Both viruses were detected in one child with low-avidity HHV-7 antibody and high-avidity HHV-6 antibody. In two children with low-avidity antibodies to both viruses, HHV-6 and HHV-7 DNAs were found, confirming dual primary infections and excluding antibody cross-reactivity.

* Corresponding author. Mailing address: Department of Virology, Royal Free and University College Medical School, Windeyer Building, 46 Cleveland St., London W1T 4JF, United Kingdom. Phone: 44 (0)20 7679 9490/9137. Fax: 44 (0)20 7580 5896. E-mail: k.n.ward{at}ucl.ac.uk.

dagger Present address: Department of Infectious Diseases & Microbiology, Imperial College School of Medicine, St. Mary's Campus, London W2 1PG, United Kingdom.

Journal of Clinical Microbiology, March 2001, p. 959-963, Vol. 39, No. 3
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.3.959-963.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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