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Journal of Clinical Microbiology, March 2001, p. 971-976, Vol. 39, No. 3
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.3.971-976.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Identification of Enteropathogenic Escherichia coli in Simian Immunodeficiency Virus-Infected Infant and Adult Rhesus Macaques

Keith G. Mansfield,1,* Kuei-Chin Lin,1 Joseph Newman,2 David Schauer,2 John MacKey,1 Andrew A. Lackner,1 and Angela Carville1

New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772,1 and Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, Massachusetts 021392

Received 5 September 2000/Returned for modification 6 December 2000/Accepted 27 December 2000

Enteropathogenic Escherichia coli (EPEC) was recognized as a common opportunistic pathogen of simian immunodeficiency virus-infected rhesus macaques (Macaca mulatta) with AIDS. Retrospective analysis revealed that 27 of 96 (28.1%) animals with AIDS had features of EPEC infection, and EPEC was the most frequent pathogen of the gastrointestinal tract identified morphologically. In 7.3% of animals dying with AIDS, EPEC represented the sole opportunistic agent of the gastrointestinal tract at death. In 20.8% of cases, it was seen in combination with one or more gastrointestinal pathogens, including Cryptosporidium parvum, Enterocytozoon bieneusi, Mycobacterium avium, Entamoeba histolytica, Balantidium coli, Strongyloides stercoralis, cytomegalovirus, and adenovirus. Clinically, infection was associated with persistent diarrhea and wasting and was more frequent in animals that died at under 1 year of age (P < 0.001, Fisher exact test). The organism was associated with the characteristic attaching and effacing lesion in colonic tissue sections and produced a focal adherence pattern on a HEp-2 assay but was negative for Shiga toxin production as assessed by PCR and a HeLa cell cytotoxicity assay. A 2.6-kb fragment encompassing the intimin gene was amplified and sequenced and revealed 99.2% identity to sequences obtained from human isolates (GenBank AF116899) corresponding to the epsilon intimin subtype. Further investigations with rhesus macaques may offer opportunities to study the impact of EPEC on AIDS pathogenesis and gastrointestinal dysfunction.

* Corresponding author. Mailing address: Harvard Medical School, New England Regional Primate Research Center, P.O. Box 9102, Southborough, MA 01772-9012. Phone: (508) 624-8183. Fax: (508) 624-8190. E-mail: Keith_Mansfield{at}HMS.Harvard.edu.

Journal of Clinical Microbiology, March 2001, p. 971-976, Vol. 39, No. 3
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.3.971-976.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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