This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Figueiredo, C.
Right arrow Articles by van Doorn, L.-J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Figueiredo, C.
Right arrow Articles by van Doorn, L.-J.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, April 2001, p. 1339-1344, Vol. 39, No. 4
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.4.1339-1344.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Assessment of Helicobacter pylori vacA and cagA Genotypes and Host Serological Response

Céu Figueiredo,1,2 Wim Quint,1 Nathalie Nouhan,1 Henk van den Munckhof,1 Paul Herbrink,3 Joost Scherpenisse,3 Wink de Boer,4 Peter Schneeberger,5 Guillermo Perez-Perez,6,7 Martin J. Blaser,6,7 and Leen-Jan van Doorn1,*

Delft Diagnostic Laboratory1 and R. de Graaf Hospital,3 Delft, Department of Internal Medicine, Bernhoven Hospital, Oss,4 and Department of Microbiology, Bosch Medicentrum, Den Bosch,5 The Netherlands; IPATIMUP and Medical Faculty, University of Porto, Porto, Portugal2; Division of Infectious Diseases, Vanderbilt University School of Medicine, Nashville, Tennessee6; and Department of Medicine, New York University School of Medicine, New York, New York7

Received 28 September 2000/Returned for modification 22 December 2000/Accepted 26 January 2001

Helicobacter pylori strains can be distinguished by genotyping of virulence-associated genes, such as vacA and cagA. Because serological discrimination between strain types would reduce the need for endoscopy, 61 patients carrying H. pylori were studied by vacA and cagA genotyping of H. pylori in gastric biopsy specimens and by detection of specific serum antibodies. Serological responses to H. pylori were determined by Helicoblot (versions 2.0 and 2.1). Antibodies to CagA also were determined by a rapid anti-CagA assay (Pyloriset screen CagA) as well as by two noncommercially developed enzyme immunoassays, each using a recombinant CagA protein. Assessment of performance of the Helicoblot assays indicated substantial interobserver variation, with kappa values between 0.20 and 0.93. There was no relationship between the serological profiles on the Helicoblot and the genotypes from the same patients, except for strong associations between the presence of anti-CagA and the cagA-positive and vacA s1 H. pylori genotypes. Detection of anti-CagA by the five different assays varied considerably, with kappa values ranging from 0.21 to 0.78. Using the cagA genotype as the "gold standard," the sensitivity and specificity of the anti-CagA assays varied from 71.4 to 85.7% and from 54.2 to 100%, respectively. Thus, serological profiles of antibodies to H. pylori are heterogeneous and, with the exception of anti-CagA antibodies, show no relation to the H. pylori vacA and cagA genotypes. Detection of anti-CagA antibodies is strongly dependent on the test used.

* Corresponding author. Mailing address: Delft Diagnostic Laboratory, R. de Graafweg 7, 2625 AD Delft, The Netherlands. Phone: 31-15-2604581. Fax: 31-15-2604550. E-mail: L.J.van.Doorn{at}ddl.nl.

Journal of Clinical Microbiology, April 2001, p. 1339-1344, Vol. 39, No. 4
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.4.1339-1344.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Veijola, L., Oksanen, A., Sipponen, P., Rautelin, H. (2008). Evaluation of a Commercial Immunoblot, Helicoblot 2.1, for Diagnosis of Helicobacter pylori Infection. CVI 15: 1705-1710 [Abstract] [Full Text]  
  • Ayala, G., Torres-Mena, J., Lopez-Carrillo, L. (2008). Association between Helicobacter pylori VacA antigens and gastric cancer depends on the detection method used: immunoblot versus neutralization of the vacuolating activity of VacA. J Med Microbiol 57: 9-14 [Abstract] [Full Text]  
  • Ghose, C., Perez-Perez, G. I., Torres, V. J., Crosatti, M., Nomura, A., Peek, R. M. Jr., Cover, T. L., Francois, F., Blaser, M. J. (2007). Serological Assays for Identification of Human Gastric Colonization by Helicobacter pylori Strains Expressing VacA m1 or m2. CVI 14: 442-450 [Abstract] [Full Text]  
  • Siman, J. H., Engstrand, L., Berglund, G., Floren, C.-H., Forsgren, A. (2005). Evaluation of Western Blot CagA Seropositivity in Helicobacter pylori-Seropositive and -Seronegative Subjects. CVI 12: 304-309 [Abstract] [Full Text]  
  • Camorlinga-Ponce, M, Romo, C, Gonzalez-Valencia, G, Munoz, O, Torres, J (2004). Topographical localisation of cagA positive and cagA negative Helicobacter pylori strains in the gastric mucosa; an in situ hybridisation study. J. Clin. Pathol. 57: 822-828 [Abstract] [Full Text]  
  • Kamangar, F., Limburg, P., Taylor, P., Dawsey, S. (2003). Re: Helicobacter pylori and Interleukin 1 Genotyping: An Opportunity to Identify High-Risk Individuals for Gastric Carcinoma. JNCI J Natl Cancer Inst 95: 760-760 [Full Text]  
  • Tomasini, M. L., Zanussi, S., Sozzi, M., Tedeschi, R., Basaglia, G., De Paoli, P. (2003). Heterogeneity of cag Genotypes in Helicobacterpylori Isolates from Human Biopsy Specimens. J. Clin. Microbiol. 41: 976-980 [Abstract] [Full Text]  
  • Rocha, A. M. C., Rocha, G. A., Santos, A., de Oliveira, C. A., Queiroz, D. M. M. (2003). Accuracy of a Commercial Enzyme-Linked Immunosorbent Assay for CagA in Patients from Brazil with and without Gastric Carcinoma. J. Clin. Microbiol. 41: 447-448 [Abstract] [Full Text]  
  • Park, C.-Y., Cho, Y.-K., Kodama, T., El-Zimaity, H. M. T., Osato, M. S., Graham, D. Y., Yamaoka, Y. (2002). New Serological Assay for Detection of Putative Helicobacter pylori Virulence Factors. J. Clin. Microbiol. 40: 4753-4756 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»