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Journal of Clinical Microbiology, April 2001, p. 1407-1415, Vol. 39, No. 4
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.4.1407-1415.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Pediatric Solid-Organ Transplant Recipients Carry Chronic Loads of Epstein-Barr Virus Exclusively in the Immunoglobulin D-Negative B-Cell Compartment

Camille Rose,1 Michael Green,2,3 Steven Webber,3 Demetrius Ellis,2 Jorges Reyes,3 and David Rowe1,*

Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh,1 and Departments of Pediatrics2 and Surgery,3 Children's Hospital of Pittsburgh, Pittsburgh, Pennsylvania 15213

Received 27 July 2000/Returned for modification 15 November 2000/Accepted 27 December 2000

Solid-organ transplant recipients are at risk for development of lymphoproliferative diseases. The purpose of this study was to examine the distribution of Epstein-Barr virus (EBV) load in the peripheral blood of pediatric transplant recipients who had become chronic viral load carriers (>8 copies/105 lymphocytes for >2 months). A total of 19 patients with viral loads ranging from 20 to 5,000 viral genome copies/105 lymphocytes were studied. Ten patients had no previous diagnosis of posttransplant lymphoproliferative disease (PT-LPD), while nine had recovered from a diagnosed case of PT-LPD. No portion of the peripheral blood viral load was detected in the cell-free plasma fraction. Viral DNA was found in a population of cells characterized as CD19hi and immunoglobulin D negative, a phenotype that is consistent with the virus being carried exclusively in the memory B-cell compartment of the peripheral blood. There was no difference in the compartmentalization based upon either the level of the viral load or the past diagnosis of an episode of PT-LPD. These results have implications for the design of tests to detect EBV infection and for the interpretation and use of positive EBV PCR assays in the management of transplant recipients.

* Corresponding author. Mailing address: Department of Infectious Diseases and Microbiology, Graduate School of Public Health, 130 DeSoto St., Pittsburgh, PA 15213. Phone: (412) 624-1529. Fax: (412) 383-7490. E-mail: rowe1+{at}pitt.edu.

Journal of Clinical Microbiology, April 2001, p. 1407-1415, Vol. 39, No. 4
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.4.1407-1415.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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