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Journal of Clinical Microbiology, April 2001, p. 1436-1442, Vol. 39, No. 4
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.4.1436-1442.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Application and Evaluation of the Interlaboratory Reproducibility of tRNA Intergenic Length Polymorphism Analysis (tDNA-PCR) for Identification of Streptococcus Species

Margo Baele,1,* Virginie Storms,2 Freddy Haesebrouck,1 Luc A. Devriese,1 Monique Gillis,2 Gerda Verschraegen,3 Thierry de Baere,3 and Mario Vaneechoutte3

Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, B-9820 Merelbeke,1 and Laboratorium voor Microbiologie (WE10V), Faculteit Wetenschappen,2 and Department of Clinical Chemistry, Microbiology and Immunology, Faculty of Medicine,3 Ghent University, B-9000 Ghent, Belgium

Received 24 October 2000/Returned for modification 13 December 2000/Accepted 31 January 2001

The discriminatory power, speed, and interlaboratory reproducibility of tRNA intergenic length polymorphism analysis (tDNA-PCR) combined with capillary electrophoresis was evaluated for the identification of streptococci. This method was carried out in three different laboratories under highly standardized conditions for 54 strains belonging to 18 different species. It was concluded that interlaboratory reproducibility of tDNA fingerprints produced by means of capillary electrophoresis was sufficiently high to permit the exchange between different laboratories and the construction of common libraries which can be consulted for comparison with fingerprints obtained independently in separate laboratories. In a second step, 17 other species were included in the study and examined in one of the participating laboratories. All Streptococcus species studied, except S. mitis, S. oralis, S. parasanguinis, S. pneumoniae, S. thermophilus, and S. vestibularis, showed distinguishable tDNA fingerprints. A database of well-characterized strains was constructed to enable computer-aided identification of unknown streptococcal isolates.

* Corresponding author. Mailing address: Laboratory of Bacteriology and Mycology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium. Phone: 32 9 264 74 34. Fax: 32 9 264 74 94. E-mail: Margo.Baele{at}rug.ac.be.

Journal of Clinical Microbiology, April 2001, p. 1436-1442, Vol. 39, No. 4
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.4.1436-1442.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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