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Journal of Clinical Microbiology, April 2001, p. 1494-1500, Vol. 39, No. 4
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.4.1494-1500.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Development of an Immunoassay for Rapid Detection of Ganglioside GM1 Mimicry in Campylobacter jejuni Strains

Martina M. Prendergast,1 Timo U. Kosunen,2 and Anthony P. Moran1,*

Department of Microbiology, National University of Ireland, Galway, Ireland,1 and Department of Bacteriology and Immunology, University of Helsinki, Finland2

Received 4 October 2000/Returned for modification 14 December 2000/Accepted 26 January 2001

Mimicry of peripheral nerve gangliosides by Campylobacter jejuni lipopolysaccharides (LPSs) has been proposed to induce cross-reacting antiganglioside antibodies in Guillain-Barré syndrome (GBS). Because current methods for LPS characterization are labor-intensive and inhibit the screening of large numbers of strains, a rapid GM1 epitope screening assay was developed. Biomass from two agar plates of confluent growth yielded sufficient LPS using a novel phenol-water and ether extraction procedure. Extracts of LPS were reacted with cholera toxin (GM1 ligand), peanut agglutinin (Galbeta 1right-arrow3GalNAc ligand), and anti-GM1 antibodies. After the assay was validated, 12 of 59 (20%) C. jejuni serostrains, including four serotypes that have not previously been associated with GBS, reacted with two or more anti-GM1 ganglioside reagents. Subsequently, LPS extracts from 5 of 7 (71%) C. jejuni isolates and 2 of 3 (67%) C. jejuni culture collection strains bore GM1 structures. Overall, the assay system was reliable, efficient, and reproducible and may be adapted for large-scale epidemiological studies.

* Corresponding author. Mailing address: Department of Microbiology, National University of Ireland, Galway, Ireland. Phone: 353-91-524411. Fax: 353-91-525700. E-mail: anthonymoran{at}nuigalway.ie.

Journal of Clinical Microbiology, April 2001, p. 1494-1500, Vol. 39, No. 4
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.4.1494-1500.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.