This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Goloubeva, V.
Right arrow Articles by Bastian, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Goloubeva, V.
Right arrow Articles by Bastian, I.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, April 2001, p. 1501-1505, Vol. 39, No. 4
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.4.1501-1505.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Evaluation of Mycobacteria Growth Indicator Tube for Direct and Indirect Drug Susceptibility Testing of Mycobacterium tuberculosis from Respiratory Specimens in a Siberian Prison Hospital

Vera Goloubeva,1 Maryvonne Lecocq,2 Piotr Lassowsky,2 Francine Matthys,3 Françoise Portaels,4 and Ivan Bastian4,5,*

Bacteriology Laboratory, Colony 33,1 and Médecins Sans Frontières,2 Mariinsk, Siberia; Médecins Sans Frontières, Brussels,3 and Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp,4 Belgium; and Institute of Medical and Veterinary Science, Adelaide, Australia5

Received 22 September 2000/Returned for modification 29 December 2000/Accepted 29 January 2001

The manual Mycobacteria Growth Indicator Tube (MGIT) method was evaluated for performing direct and indirect drug susceptibility testing (DST) of Mycobacterium tuberculosis for isoniazid and rifampin on 101 strongly smear-positive sputum specimens in a Siberian prison hospital. Using the indirect method of proportion (MOP) as the "gold standard," the accuracies of isoniazid and rifampin susceptibility testing by the direct MGIT system were 97.0 and 94.1%, respectively. The accuracy of the indirect MGIT system was 98.0% for both drugs. The turnaround times from specimen processing to reporting of the DST results ranged between 4 and 23 (mean, 9.2) days by the direct MGIT method, 9 and 30 (mean, 15.3) days by the indirect MGIT method, and 26 and 101 (mean, 59.6) days by the indirect MOP. MGIT appears to be a reliable, rapid, and convenient method for performing direct and indirect DSTs in low-resource settings, but further studies are required to refine the direct DST protocol. Cost is the only factor prohibiting widespread implementation of MGIT.

* Corresponding author. Mailing address: Institute of Medical & Veterinary Science, P.O. Box 14, Rundle Mall, Adelaide, SA 5000, Australia. Phone: 61-8-8222-3291. Fax: 61-8-8222-3543. E-mail: ivan.bastian{at}imvs.sa.gov.au.

Journal of Clinical Microbiology, April 2001, p. 1501-1505, Vol. 39, No. 4
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.4.1501-1505.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Affolabi, D., Odoun, M., Sanoussi, N., Martin, A., Palomino, J. C., Kestens, L., Anagonou, S., Portaels, F. (2008). Rapid and Inexpensive Detection of Multidrug-Resistant Mycobacterium tuberculosis with the Nitrate Reductase Assay Using Liquid Medium and Direct Application to Sputum Samples. J. Clin. Microbiol. 46: 3243-3245 [Abstract] [Full Text]  
  • Shiferaw, G., Woldeamanuel, Y., Gebeyehu, M., Girmachew, F., Demessie, D., Lemma, E. (2007). Evaluation of Microscopic Observation Drug Susceptibility Assay for Detection of Multidrug-Resistant Mycobacterium tuberculosis. J. Clin. Microbiol. 45: 1093-1097 [Abstract] [Full Text]  
  • Huang, T.-S., Liu, Y.-C., Tu, H.-Z., Sy, C.-L., Chen, Y.-S., Chen, B.-C. (2007). Rapid Purity Check Method for Susceptibility Testing of M. tuberculosis Complex with the MGIT 960 System. Annals of Clinical & Laboratory Science 37: 323-329 [Abstract] [Full Text]  
  • Nateche, F., Martin, A., Baraka, S., Palomino, J. C., Khaled, S., Portaels, F. (2006). Application of the resazurin microtitre assay for detection of multidrug resistance in Mycobacterium tuberculosis in Algiers.. J Med Microbiol 55: 857-860 [Abstract] [Full Text]  
  • Somoskovi, A., Clobridge, A., Larsen, S. C., Sinyavskiy, O., Surucuoglu, S., Parsons, L. M., Salfinger, M. (2006). Does the MGIT 960 System Improve the Turnaround Times for Growth Detection and Susceptibility Testing of the Mycobacterium tuberculosis Complex?. J. Clin. Microbiol. 44: 2314-2315 [Full Text]  
  • Rusch-Gerdes, S., Pfyffer, G. E., Casal, M., Chadwick, M., Siddiqi, S. (2006). Multicenter Laboratory Validation of the BACTEC MGIT 960 Technique for Testing Susceptibilities of Mycobacterium tuberculosis to Classical Second-Line Drugs and Newer Antimicrobials.. J. Clin. Microbiol. 44: 688-692 [Abstract] [Full Text]  
  • Musa, H. R., Ambroggi, M., Souto, A., Angeby, K. A. K. (2005). Drug Susceptibility Testing of Mycobacterium tuberculosis by a Nitrate Reductase Assay Applied Directly on Microscopy-Positive Sputum Samples. J. Clin. Microbiol. 43: 3159-3161 [Abstract] [Full Text]  
  • Huang, T.-S., Lee, S. S.-J., Tu, H.-Z., Huang, W.-K., Chen, Y.-S., Huang, C.-K., Wann, S.-R., Lin, H.-H., Liu, Y.-C. (2004). Use of MGIT 960 for rapid quantitative measurement of the susceptibility of Mycobacterium tuberculosis complex to ciprofloxacin and ethionamide. J Antimicrob Chemother 53: 600-603 [Abstract] [Full Text]  
  • Abate, G., Aseffa, A., Selassie, A., Goshu, S., Fekade, B., WoldeMeskal, D., Miorner, H. (2004). Direct Colorimetric Assay for Rapid Detection of Rifampin-Resistant Mycobacterium tuberculosis. J. Clin. Microbiol. 42: 871-873 [Abstract] [Full Text]  
  • Syre, H., Phyu, S., Sandven, P., Bjorvatn, B., Grewal, H. M. S. (2003). Rapid Colorimetric Method for Testing Susceptibility of Mycobacterium tuberculosis to Isoniazid and Rifampin in Liquid Cultures. J. Clin. Microbiol. 41: 5173-5177 [Abstract] [Full Text]  
  • Bemer, P., Palicova, F., Rusch-Gerdes, S., Drugeon, H. B., Pfyffer, G. E. (2002). Multicenter Evaluation of Fully Automated BACTEC Mycobacteria Growth Indicator Tube 960 System for Susceptibility Testing of Mycobacterium tuberculosis. J. Clin. Microbiol. 40: 150-154 [Abstract] [Full Text]  
  • Bastian, I., Rigouts, L., Palomino, J. C., Portaels, F. (2001). Kanamycin Susceptibility Testing of Mycobacterium tuberculosis Using Mycobacterium Growth Indicator Tube and a Colorimetric Method. Antimicrob. Agents Chemother. 45: 1934-1936 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»