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Journal of Clinical Microbiology, April 2001, p. 1506-1509, Vol. 39, No. 4
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.4.1506-1509.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Diagnosis and Monitoring of Murine Histoplasmosis by a Nested PCR Assay

Ralf Bialek,1,* Jörg Fischer,1 Antje Feucht,1 Laura K. Najvar,2 Klaus Dietz,3 Jürgen Knobloch,1 and John R. Graybill2,4

Institute for Tropical Medicine1 and Department of Medical Biometry,3 University Hospital Tübingen, Tübingen, Germany; and University of Texas Health Science Center at San Antonio2 and Audie Murphy VA Hospital,4 San Antonio, Texas

Received 20 November 2000/Returned for modification 8 January 2001/Accepted 5 February 2001

A newly developed nested PCR assay was applied to murine models of histoplasmosis. ICR and BALB/c mice were intravenously infected with Histoplasma capsulatum and sacrificed up to 29 days later. Samples of blood, spleen, and lung homogenates were cultured and examined by the PCR assay. In the ICR mouse model, 265 of 319 organ samples showed concordant results. With 7 samples, the culture was positive and the PCR assay was negative whereas a positive PCR but a negative culture were obtained with 47 samples (P < 0.0001 according to McNemar's test). Organ homogenates and blood samples of either spontaneously cured or treated BALB/c mice were PCR negative. The nested PCR assay performs excellently in the monitoring of spontaneously and treatment-cured murine histoplasmosis. It limits the infection risks of the laboratory staff and might be of diagnostic value for humans.

* Corresponding author. Mailing address: Institut für Tropenmedizin, Universitätsklinikum Tübingen, Keplerstrasse 15, 72074 Tübingen, Germany. Phone: 49 7071-298 2367. Fax: 49 7071 29 5267. E-mail: ralf.bialek{at}med.uni-tuebingen.de.

Journal of Clinical Microbiology, April 2001, p. 1506-1509, Vol. 39, No. 4
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.4.1506-1509.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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