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Journal of Clinical Microbiology, April 2001, p. 1549-1552, Vol. 39, No. 4
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.4.1549-1552.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

16S Ribosomal DNA Sequence Analysis Distinguishes Biotypes of Streptococcus bovis: Streptococcus bovis Biotype II/2 Is a Separate Genospecies and the Predominant Clinical Isolate in Adult Males

Jill E. Clarridge III,1,2,3,* Silvia M. Attorri,1,3 Qing Zhang,1,3 and John Bartell4

Department of Pathology1 and Department of Molecular Virology and Microbiology,2 Baylor College of Medicine, and Pathology and Laboratory Medicine Service, Veterans Affairs Medical Center,3 Houston, Texas, and MIDI, Inc., Newark, Delaware4

Received 25 October 2000/Returned for modification 19 December 2000/Accepted 27 January 2001

We characterized 22 human clinical strains of Streptococcus bovis by genotypic (16S rRNA gene sequence analysis [MicroSeq]; Applied Biosystems, Foster City, Calif.) and phenotypic (API 20 Strep and Rapid ID32 Strep systems (bioMerieux Vitek, Hazelton, Mo.) methods. The strains, isolated from blood, cerebrospinal fluid (CSF), and urine, formed two distinct 16S ribosomal DNA sequence clusters. Three strains which were associated with endocarditis urinary tract infection (UTI), and sepsis clustered with the S. bovis type strain ATCC 33317 (cluster 1); other closely related type strains were S. equinus and S. infantarius. Nineteen strains clustered at a distance of about 2.5% dissimilarity to the S. bovis type strain (cluster 2) and were associated with central nervous system (CNS) disease in addition to endocarditis, UTI, and sepsis. All strains were distinct from S. gallolyticus. Within cluster 2, a single strain grouped with ATCC strain 43143 (cluster 2a) and may be phenotypically distinct. All the other strains formed a second subgroup (cluster 2b) that was biochemically similar to S. bovis biotype II/2 (mannitol negative and beta galactosidase, alpha galactosidase, beta glucuronidase, and trehalose positive). The API 20 Strep system identified isolates of cluster 2b as S. bovis biotype II/2, those of cluster 1 as S. bovis biotype II/1, and that of cluster 2a as S. bovis biotype I. There was an excellent correlation of biotype and genotype: S. bovis biotype II/2 isolates form a separate genospecies distinct from the S. bovis, S. gallolyticus, and S. infantarius type strains and are the most common isolates in adult males.

* Corresponding author. Mailing address: Pathology and Laboratory Medicine Services (113), VA Medical Center and Baylor College of Medicine, 2002 Holcombe Blvd., Houston, TX 77030. Phone: (713) 794-7336. Fax: (713) 794-7657. E-mail: jillc{at}bcm.tmc.edu.

Journal of Clinical Microbiology, April 2001, p. 1549-1552, Vol. 39, No. 4
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.4.1549-1552.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.