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Journal of Clinical Microbiology, April 2001, p. 1591-1594, Vol. 39, No. 4
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.4.1591-1594.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

The Susceptibility of Mycobacterium tuberculosis to Isoniazid and the Argright-arrow Leu Mutation at Codon 463 of katG Are Not Associated

H. R. van Doorn,1,* E. J. Kuijper,1 A. van der Ende,1 A. G. A. Welten,2 D. van Soolingen,3 P. E. W. de Haas,3 and J. Dankert1

Academic Medical Center, Department of Medical Microbiology,1 and Free University of Amsterdam, Department of Cell Biology, Faculty of Medicine,2 Amsterdam, and National Institute of Public Health and Environment, Diagnostic Laboratory for Infectious Disease and Perinatal Screening, Bilthoven,3 The Netherlands

Received 9 October 2000/Returned for modification 14 December 2000/Accepted 4 February 2001

A mutation (CCGright-arrowCTG [Argright-arrowLeu]) in codon 463 of katG (catalase peroxidase) of Mycobacterium tuberculosis has been found in isoniazid (INH)-resistant strains. A PCR restriction endonuclease analysis to detect this mutation was applied to 395 M. tuberculosis isolates from patients in The Netherlands. The proportion of isolates with a detectable mutation was 32% (32 out of 100) and 29% (85 out of 295) among INH-susceptible isolates and INH-resistant or -intermediate isolates, respectively. Sequencing of five INH-susceptible isolates with such mutations showed that all five had the Arg463Leu mutation. We conclude that the Arg463Leu mutation of katG of M. tuberculosis is not a reliable indicator of INH resistance.

* Corresponding author. Mailing address: Academic Medical Center, Dept. of Medical Microbiology, P.O. Box 22700, 1100DE Amsterdam, The Netherlands. Phone: 31 20 5664860. Fax: 31 20 6979271. E-mail: h.r.vandoorn{at}amc.uva.nl.

Journal of Clinical Microbiology, April 2001, p. 1591-1594, Vol. 39, No. 4
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.4.1591-1594.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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