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Journal of Clinical Microbiology, April 2001, p. 1618-1621, Vol. 39, No. 4
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.4.1618-1621.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Evaluation of PCR Methods for Rapid Identification and Differentiation of Streptococcus uberis and Streptococcus parauberis

A. A. Hassan, I. U. Khan, A. Abdulmawjood, and C. Lämmler*

Institut für Tierärztliche Nahrungsmittelkunde, Bakteriologie und Hygiene der Milch, der Justus-Liebig-Universität Gießen, Giessen, Germany

Received 4 October 2000/Returned for modification 29 December 2000/Accepted 6 February 2001

Streptococcus uberis and Streptococcus parauberis reference strains and isolates obtained from routine diagnostics were investigated by PCR with oligonucleotide primers designed according to species-specific parts of the 16S rRNA gene, the 23S rRNA gene, and the 16S-23S rRNA intergenic spacer region of both species. All three primer pairs allowed an identification of 67 isolates as S. uberis and 4 isolates as S. parauberis.

* Corresponding author. Mailing address: Institut für Tierärztliche Nahrungsmittelkunde, Bakteriologie und Hygiene der Milch, Justus-Liebig-Universität Gießen, Frankfurter Str. 107, 35392 Gießen, Germany. Phone: 49641-9938383. Fax: 49641-9938389. E-mail: Christoph.Laemmler{at}vetmed.uni-giessen.de.

Journal of Clinical Microbiology, April 2001, p. 1618-1621, Vol. 39, No. 4
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.4.1618-1621.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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