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Journal of Clinical Microbiology, April 2001, p. 1622-1625, Vol. 39, No. 4
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.4.1622-1625.2001

Random Amplified Polymorphic DNA Assay as a Rapid Tool in Screening for Neisseria meningitidis Serogroup C Isolates of Electrophoretic Type 24

Susanna Schmink,1,* Michael W. Reeves,1 Brian Plikaytis,2 and Tanja Popovic1

Meningitis and Special Pathogens Branch1 and Biostatistics and Information Management Branch,2 Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333

Received 20 October 2000/Returned for modification 17 December 2000/Accepted 29 January 2001

Neisseria meningitidis serogroup C (NMSC) isolates of electrophoretic type 24 (ET-24), as identified by multilocus enzyme electrophoresis, are the main cause of serogroup C meningococcal disease outbreaks and sporadic meningococcal disease in the United States. We evaluated a random amplified polymorphic DNA assay as a rapid tool to screen for isolates of ET-24 by testing 199 NMSC isolates of 51 different ETs. A sensitivity of 88% and a specificity of 87% was achieved in identification of ET-24 isolates when the patterns obtained by two primers, P1 and P5, were analyzed together.

* Corresponding author. Mailing address: Epidemic Investigations Laboratory, Meningitis and Special Pathogens Branch, Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Bldg. 5, Room MS D11, 1600 Clifton Rd., NE, Atlanta, GA 30333. Phone: (404) 639-1380. Fax: (404) 639-4421. E-mail: zma6{at}cdc.gov.

Journal of Clinical Microbiology, April 2001, p. 1622-1625, Vol. 39, No. 4
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.4.1622-1625.2001




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