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Journal of Clinical Microbiology, April 2001, p. 1626-1629, Vol. 39, No. 4
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.4.1626-1629.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Nucleic Acid Sequence-Based Amplification of Aspergillus RNA in Blood Samples

Juergen Loeffler,1,* Holger Hebart,1 Philipp Cox,1 Nicole Flues,1 Ulrike Schumacher,2 and Hermann Einsele1

Medizinische Klinik, Abteilung II, Eberhard-Karls-Universität Tübingen,1 and Institut für Medizinische Mikrobiologie,2 72076 Tübingen, Germany

Received 13 November 2000/Returned for modification 12 December 2000/Accepted 29 January 2001

Nucleic acid sequence-based amplification (NASBA), an isothermal amplification technique, was established and evaluated for the detection of Aspergillus RNA and compared with a previously published, well-defined real-time PCR assay amplifying a region of the Aspergillus 18S rRNA gene. NASBA showed a lower detection limit of 1 CFU and detected RNA from five different clinically relevant Aspergillus species, including Aspergillus fumigatus. All 77 blood samples tested by PCR and NASBA showed identical results in both assays. Results with the NASBA technique were obtained within 6 h. Thus, the NASBA technique provided a valuable tool for sensitive, specific, fast, and reliable detection of Aspergillus RNA with potential for routine diagnosis, including the possibility to test the viability of cells.

* Corresponding author. Mailing address: Medizinische Klinik, Abt. II, Labor Prof. Dr. Med. H. Einsele, Otfried-Mueller-Str. 10, 72076 Tubingen, Germany. Phone: 49-7071-2987355. Fax: 49-7071-293179. E-mail: juergen.loeffler{at}med.uni-tuebingen.de.

Journal of Clinical Microbiology, April 2001, p. 1626-1629, Vol. 39, No. 4
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.4.1626-1629.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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