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Journal of Clinical Microbiology, April 2001, p. 1633-1637, Vol. 39, No. 4
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.4.1633-1637.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

B-Cell Epitope Mapping of the VapA Protein of Rhodococcus equi: Implications for Early Detection of R. equi Disease in Foals

Thiru Vanniasinkam,1 Mary D. Barton,1,2 and Michael W. Heuzenroeder2,*

School of Pharmacy and Medical Sciences, University of South Australia,1 and Infectious Diseases Laboratories, Institute of Medical and Veterinary Science,2 Adelaide 5000, Australia

Received 9 October 2000/Returned for modification 19 December 2000/Accepted 29 January 2001

Linear B-cell epitopes of the Rhodococcus equi virulence-associated protein (VapA) were mapped using a synthetic peptide bank in this study. The peptides were screened in an enzyme-linked immunosorbent assay (ELISA) with a total of 70 sera from foals with current R. equi disease (51 sera), as well as from foals that had either recovered from R. equi infection 10 months previously (3 sera) or that had no known history of R. equi disease (16 sera). An epitope with the sequence NLQKDEPNGRA was identified and was universally recognized by all 51 sera from foals with R. equi disease and was not recognized by any of the other sera. There was poor reactivity between all sera and peptides relating to other areas of the VapA protein. It is proposed that an ELISA based upon a defined peptide epitope may be used in an improved serological diagnostic test for R. equi infection in foals.

* Corresponding author. Mailing Address: Infectious Diseases Laboratories, Institute of Medical and Veterinary Science, Adelaide 5000, Australia. Phone: (08) 8222-3275. Fax: (08) 8222-3543. E-mail: heuzenroeder{at}imvs.sa.gov.au.

Journal of Clinical Microbiology, April 2001, p. 1633-1637, Vol. 39, No. 4
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.4.1633-1637.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Torres, D., Espino, A. M. (2006). Mapping of B-Cell Epitopes on a Novel 11.5-Kilodalton Fasciola hepatica-Schistosoma mansoni Cross-Reactive Antigen Belonging to a Member of the F. hepatica Saposin-Like Protein Family.. Infect. Immun. 74: 4932-4938 [Abstract] [Full Text]  


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