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Journal of Clinical Microbiology, April 2001, p. 1644-1646, Vol. 39, No. 4
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.4.1644-1646.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Genetic Variation among Human Isolates of Uninucleated Cyst-Producing Entamoeba Species

Jaco J. Verweij,1,* Anton M. Polderman,1 and C. Graham Clark2

Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands,1 and Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, England2

Received 21 September 2000/Returned for modification 6 November 2000/Accepted 4 February 2001

Twelve human infections with Entamoeba spp. producing uninucleated cysts were studied. DNA was extracted from infected feces and used to amplify part of the ameba small-subunit rRNA gene. Sequence analysis identified four distinct types of Entamoeba, all of which are related to Entamoeba polecki and E. chattoni and two of which have not been reported previously. Whether these genetic types represent different species is unclear. We propose that the agent of all human infections with uninucleated cyst-producing Entamoeba species be reported as "E. polecki-like."

* Corresponding author. Mailing address: Department of Parasitology, Leiden University Medical Center, P.O. Box 9600, Leiden, The Netherlands. Phone: 31-71-5265080. Fax: 31-71-5266907. E-mail: j.j.verweij{at}LUMC.nl.

Journal of Clinical Microbiology, April 2001, p. 1644-1646, Vol. 39, No. 4
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.4.1644-1646.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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