Group A Streptococcal Genotypes from Pediatric Throat Isolates in Rome, Italy

doi:10.1128/JCM.39.5.1687-1690.2001

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Journal of Clinical Microbiology, May 2001, p. 1687-1690, Vol. 39, No. 5
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.5.1687-1690.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Group A Streptococcal Genotypes from Pediatric Throat Isolates in Rome, Italy

Giordano Dicuonzo,¹ Giovanni Gherardi,¹ Giulia Lorino,² Silvia Angeletti,¹ Marina De Cesaris,¹ Ersilia Fiscarelli,³ Debra E. Bessen,⁴ and Bernard Beall⁵^,^*

Department of Medicine and Microbiology, Università Campus Bio-Medico,¹ Department of Microbiology, Università La Sapienza,² and Laboratory of Microbiology, Pediatric Hospital "Bambin Gesù,"³ Rome, Italy; Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, Connecticut 06520⁴; and Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333⁵

Received 18 December 2000/Returned for modification 27 January 2001/Accepted 17 February 2001

In a study assessing genetic diversity, 114 group A streptococcus (GAS) isolates were recovered from pediatric pharyngitispatients in Rome, Italy. These isolates comprised 22 differentM protein gene (emm) sequence types, 14 of which were associatedwith a distinct serum opacity factor/fibronectin binding proteingene (sof) sequence type. Isolates with the same emm gene sequencetype generally shared a highly conserved chromosomal macrorestrictionprofile. In three instances, isolates with dissimilar macrorestrictionprofiles had identical emm types; in each of these cases multilocussequence typing revealed that isolates with the same emm typewere clones having the same allelic profiles. Ninety-eight percentof the pharyngeal isolates had emm types previously found to behighly associated with mga locus gene patterns commonly foundin pharyngeal GASisolates.

^* Corresponding author. Mailing address: Centers for Disease Control and Prevention, Mailstop C02, 1600 Clifton Rd., NE, Atlanta,GA 30333. Phone: (404) 639-1237. Fax: (404) 639-3123. E-mail:BBeall{at}cdc.gov.

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