This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Van Dyck, E.
Right arrow Articles by Laga, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Van Dyck, E.
Right arrow Articles by Laga, M.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, May 2001, p. 1751-1756, Vol. 39, No. 5
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.5.1751-1756.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Detection of Chlamydia trachomatis and Neisseria gonorrhoeae by Enzyme Immunoassay, Culture, and Three Nucleic Acid Amplification Tests

E. Van Dyck,1,* M. Ieven,2 S. Pattyn,1 L. Van Damme,1 and M. Laga1

STD/HIV Research and Intervention Unit, Department of Microbiology, Institute of Tropical Medicine,1 and Department of Microbiology, University Hospital,2 Antwerp, Belgium

Received 3 November 2000/Returned for modification 16 January 2001/Accepted 6 February 2001

The purpose of this study was to evaluate and compare three commercially available nucleic acid amplification tests (NAATs) for the detection of Neisseria gonorrhoeae and Chlamydia trachomatis. Roche PCR and Becton Dickinson strand displacement amplification (SDA) were performed on 733 endocervical swab specimens from commercial sex workers. Abbott ligase chain reaction (LCR) was performed on a subset of 396 samples. Endocervical specimens from all women were also tested by culture for N. gonorrhoeae and by Syva MicroTrak enzyme immunoassay (EIA) for C. trachomatis. A positive N. gonorrhoeae result was defined as a positive result by culture or by two NAATs, and a positive C. trachomatis result was defined as a positive result by two tests. According to these definitions, the sensitivities and specificities for the subsample of 396 specimens of N. gonorrhoeae culture, PCR, SDA, and LCR were 69.8, 95.2, 88.9, and 88.9% and 100, 99.4, 100, and 99.1%, respectively; the sensitivities and specificities of C. trachomatis EIA, PCR, SDA, and LCR were 42.0, 98.0, 94.0, and 90.0% and 100, 98.0, 100, and 98.6%, respectively. The performance characteristics of N. gonorrhoeae culture, PCR, and SDA and C. trachomatis EIA, PCR, and SDA for all 733 specimens were defined without inclusion of LCR results and by discrepant analysis after resolution of discordant N. gonorrhoeae PCR results and of discordant C. trachomatis EIA and PCR results by LCR testing. The sensitivities of N. gonorrhoeae culture, PCR, and SDA before and after LCR resolution were 67.8, 95.7, and 93.9% and 65, 95.8, and 90.0%, respectively. The sensitivities of C. trachomatis EIA, PCR, and SDA decreased from 39.4, 100, and 100% to 38.7, 98.7, and 94.7%, respectively. All three NAATs proved to be superior to N. gonorrhoeae culture and to C. trachomatis EIA. The accuracies of the different NAATs were quite similar. SDA was the only amplification assay with 100% specificity for detection of both N. gonorrhoeae and C. trachomatis in endocervical specimens.

* Corresponding author. Mailing address: STD/HIV Research and Intervention Unit, Department of Microbiology, Institute of Tropical Medicine, Nationalestraat 155, B-2000 Antwerp, Belgium. Phone: 32 3 247 63 29. Fax: 32 3 247 63 33. E-mail: evandyck{at}itg.be.

Journal of Clinical Microbiology, May 2001, p. 1751-1756, Vol. 39, No. 5
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.5.1751-1756.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Bignell, C (2009). 2009 European (IUSTI/WHO) Guideline on the Diagnosis and Treatment of Gonorrhoea in Adults. Int J STD AIDS 20: 453-457 [Full Text]  
  • Mahto, M, Zia, S, Ritchie, D, Mallinson, H (2009). Diagnosis, management and prevalence estimation of gonorrhoea: influences of Aptima Combo 2 assay with alternative target confirmation. Int J STD AIDS 20: 315-319 [Abstract] [Full Text]  
  • Meader, E, Waters, J, Sillis, M (2008). Chlamydia trachomatis RNA in the environment: is there potential for false-positive nucleic acid amplification test results?. Sex. Transm. Infect. 84: 107-110 [Abstract] [Full Text]  
  • Ryan, C., Kudesia, G., McIntyre, S., Davies, S., Zadik, P., Kinghorn, G. R, Bignell, C J (2007). BD ProbeTec ET assay for the diagnosis of gonorrhoea in a high-risk population: a protocol for replacing traditional microscopy and culture techniques. Sex. Transm. Infect. 83: 175-179 [Abstract] [Full Text]  
  • Mangold, K. A., Regner, M., Tajuddin, M., Tajuddin, A. M., Jennings, L., Du, H., Kaul, K. L. (2007). Neisseria Species Identification Assay for the Confirmation of Neisseria gonorrhoeae-Positive Results of the COBAS Amplicor PCR. J. Clin. Microbiol. 45: 1403-1409 [Abstract] [Full Text]  
  • Marshall, R., Chernesky, M., Jang, D., Hook, E. W., Cartwright, C. P., Howell-Adams, B., Ho, S., Welk, J., Lai-Zhang, J., Brashear, J., Diedrich, B., Otis, K., Webb, E., Robinson, J., Yu, H. (2007). Characteristics of the m2000 Automated Sample Preparation and Multiplex Real-Time PCR System for Detection of Chlamydia trachomatis and Neisseria gonorrhoeae. J. Clin. Microbiol. 45: 747-751 [Abstract] [Full Text]  
  • Jalal, H., Al-Suwaine, A., Stephen, H., Carne, C., Sonnex, C. (2007). Comparative performance of the Roche COBAS Amplicor assay and an in-house real-time PCR assay for diagnosis of Chlamydia trachomatis infection. J Med Microbiol 56: 320-322 [Abstract] [Full Text]  
  • Bignell, C, Ison, C A, Jungmann, E (2006). Gonorrhoea. Sex. Transm. Infect. 82: iv6-iv9 [Full Text]  
  • Schachter, J., Chow, J. M., Howard, H., Bolan, G., Moncada, J. (2006). Detection of Chlamydia trachomatis by Nucleic Acid Amplification Testing: Our Evaluation Suggests that CDC-Recommended Approaches for Confirmatory Testing Are Ill-Advised.. J. Clin. Microbiol. 44: 2512-2517 [Abstract] [Full Text]  
  • Chernesky, M., Jang, D., Luinstra, K., Chong, S., Smieja, M., Cai, W., Hayhoe, B., Portillo, E., MacRitchie, C., Main, C., Ewert, R. (2006). High Analytical Sensitivity and Low Rates of Inhibition May Contribute to Detection of Chlamydia trachomatis in Significantly More Women by the APTIMA Combo 2 Assay. J. Clin. Microbiol. 44: 400-405 [Abstract] [Full Text]  
  • Whiley, D. M., Tapsall, J. W., Sloots, T. P. (2006). Nucleic Acid Amplification Testing for Neisseria gonorrhoeae: An Ongoing Challenge. J. Mol. Diagn. 8: 3-15 [Abstract] [Full Text]  
  • Tebb, K. P., Pantell, R. H., Wibbelsman, C. J., Neuhaus, J. M., Tipton, A. C., Pecson, S. C., Pai-Dhungat, M., Ko, T. H., Shafer, M.-A. B. (2005). Screening Sexually Active Adolescents for Chlamydia trachomatis : What About the Boys?. Am. J. Public Health 95: 1806-1810 [Abstract] [Full Text]  
  • Lynn, F., Hobbs, M. M., Zenilman, J. M., Behets, F. M. T. F., Van Damme, K., Rasamindrakotroka, A., Bash, M. C. (2005). Genetic Typing of the Porin Protein of Neisseria gonorrhoeae from Clinical Noncultured Samples for Strain Characterization and Identification of Mixed Gonococcal Infections. J. Clin. Microbiol. 43: 368-375 [Abstract] [Full Text]  
  • Boyadzhyan, B., Yashina, T., Yatabe, J. H., Patnaik, M., Hill, C. S. (2004). Comparison of the APTIMA CT and GC Assays with the APTIMA Combo 2 Assay, the Abbott LCx Assay, and Direct Fluorescent-Antibody and Culture Assays for Detection of Chlamydia trachomatis and Neisseria gonorrhoeae. J. Clin. Microbiol. 42: 3089-3093 [Abstract] [Full Text]  
  • Shafer, M.-A., Moncada, J., Boyer, C. B., Betsinger, K., Flinn, S. D., Schachter, J. (2003). Comparing First-Void Urine Specimens, Self-Collected Vaginal Swabs, and Endocervical Specimens To Detect Chlamydia trachomatis and Neisseria gonorrhoeae by a Nucleic Acid Amplification Test. J. Clin. Microbiol. 41: 4395-4399 [Abstract] [Full Text]  
  • Verhoeven, V, Avonts, D, Meheus, A, Goossens, H, Ieven, M, Chapelle, S, Lammens, C, Van Royen, P (2003). Chlamydial infection: an accurate model for opportunistic screening in general practice. Sex. Transm. Infect. 79: 313-317 [Abstract] [Full Text]  
  • Vinayagamoorthy, T., Mulatz, K., Hodkinson, R. (2003). Nucleotide Sequence-Based Multitarget Identification. J. Clin. Microbiol. 41: 3284-3292 [Abstract] [Full Text]  
  • Shafer, M.-A. B., Tebb, K. P., Pantell, R. H., Wibbelsman, C. J., Neuhaus, J. M., Tipton, A. C., Kunin, S. B., Ko, T. H., Schweppe, D. M., Bergman, D. A. (2002). Effect of a Clinical Practice Improvement Intervention on Chlamydial Screening Among Adolescent Girls. JAMA 288: 2846-2852 [Abstract] [Full Text]  
  • Diemert, D. J., Libman, M. D., Lebel, P. (2002). Confirmation by 16S rRNA PCR of the COBAS AMPLICOR CT/NG Test for Diagnosis of Neisseria gonorrhoeae Infection in a Low-Prevalence Population. J. Clin. Microbiol. 40: 4056-4059 [Abstract] [Full Text]  
  • Van Der Pol, B., Williams, J. A., Smith, N. J., Batteiger, B. E., Cullen, A. P., Erdman, H., Edens, T., Davis, K., Salim-Hammad, H., Chou, V. W., Scearce, L., Blutman, J., Payne, W. J. (2002). Evaluation of the Digene Hybrid Capture II Assay with the Rapid Capture System for Detection of Chlamydia trachomatis and Neisseria gonorrhoeae. J. Clin. Microbiol. 40: 3558-3564 [Abstract] [Full Text]  
  • Chernesky, M A (2002). Chlamydia trachomatis diagnostics. Sex. Transm. Infect. 78: 232-234 [Abstract] [Full Text]  
  • Zhang, W., Cohenford, M., Lentrichia, B., Isenberg, H. D., Simson, E., Li, H., Yi, J., Zhang, D. Y. (2002). Detection of Chlamydia trachomatis by Isothermal Ramification Amplification Method: a Feasibility Study. J. Clin. Microbiol. 40: 128-132 [Abstract] [Full Text]  
  • Van Dyck, E., Smet, H., Van Damme, L., Laga, M. (2001). Evaluation of the Roche Neisseria gonorrhoeae 16S rRNA PCR for Confirmation of AMPLICOR PCR-Positive Samples and Comparison of Its Diagnostic Performance According to Storage Conditions and Preparation of Endocervical Specimens. J. Clin. Microbiol. 39: 2280-2282 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»